Rabbit RSK2 Polyclonal Antibody | anti-RPS6KA3 antibody

Anti-RSK2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101100

• Gene Names: Rps6ka3; Rsk2; MPK-9; pp90RSK2; S6K-alpha3
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: RSK2; Polyclonal Antibody; Anti-RSK2 Antibody; Rabbit Polyclonal; RSK2 Antibody; Rabbit PAb; Antigen Affinity Purified; ribosomal protein S6 kinase; 90kDa; polypeptide 3; Anti-CLS Antibody; Anti-HU-3 Antibody; Anti-ISPK-1 Antibody; Anti-MAPKAPK1B Antibody; Anti-MRX19 Antibody; Anti-p90-RSK2 Antibody; Anti-pp90RSK2 Antibody; Anti-RSK Antibody; Anti-S6K-alpha3 Antibody; anti-RPS6KA3 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human RSK2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-RPS6KA3 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:300-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human RSK2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human RSK2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-RPS6KA3 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: NIH3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:84 kDa Observed band size:88 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of RPS6KA3 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human RPS6KA3 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.)

Immunoprecipitation (IP)

(RPS6KA3 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg HepG2 Whole Cell Lysate Lane C:0.5 mg NIH3T3 Whole Cell Lysate 4 uL anti-RPS6KA3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-RPS6KA3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 84 kDa Observed band size: 88 kDa)

Related Product Information for anti-RPS6KA3 antibody

Coffin-Lowry syndrome (CLS) is a rare X-linked dominant disorder characterized by intellectual disability, craniofacial abnormalities, short stature, tapering fingers, hypotonia, and skeletal malformations. CLS is caused by mutations in the Ribosomal Protein S6 Kinase, 90 kDa, Polypeptide 3 (RPS6KA3) gene located at Xp22.12, which encodes Ribosomal S6 Kinase 2 (RSK2). This gene encodes for a growth factor-regulated serine/threonine-protein kinase, RSK2 (ribosomal S6 kinase 2), acting in the Ras-mitogen-activated protein kinase signaling pathway. Mutations in the RPS6KA3 gene are extremely heterogeneous and lead to premature termination of translation and/or to loss of phosphotransferase activity of the RSK2 protein. Screening for RSK2 mutations is essential in most cases to confirm the identification as well as for genetic counseling. This gene also affects nonsyndromic X-linked ID and nonsyndromic X-linked ID without bony abnormalities.

References

Nishimoto HK, et al. (2014) The historical coffin-lowry syndrome family revisited: Identification of two novel mutations of rps6ka3 in three male patients. Am J Med Genet A 164a (9): 2172-2179.Matsumoto A, et al. (2013) An xp22.12 microduplication including rps6ka3 identified in a family with variably affected intellectual and behavioral disabilities. J Hum Genet 58 (11): 755-757.Delaunoy JP, et al. (2006) Identification of novel mutations in the rsk2 gene (rps6ka3) in patients with coffin-lowry syndrome. Clin Genet 70 (2): 161-166.

NCBI and Uniprot Product Information

• NCBI GI #: 22507357
• NCBI GeneID: 110651
• NCBI Accession #: NP_683747.1
• NCBI GenBank Nucleotide #: NM_148945.1
• UniProt Accession #: P18654; Q03140; Q8K3J8; B1AXN4
• Molecular Weight: 83,694 Da
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-3
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase polypeptide 3
• NCBI Official Symbol: Rps6ka3
• NCBI Official Synonym Symbols: Rsk2; MPK-9; pp90RSK2; S6K-alpha3
• NCBI Protein Information: ribosomal protein S6 kinase alpha-3; RSK-2; p90RSK3; p90-RSK 3; MAPKAPK-1b; S6K-alpha-3; MAPKAP kinase 1b; ribosomal S6 kinase 2; ribosomal protein S6 kinase 2; MAPK-activated protein kinase 1b; 90 kDa ribosomal protein S6 kinase 3; MAP kinase-activated p
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-3
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: Rps6ka3
• UniProt Synonym Gene Names: Mapkapk1b; Rps6ka-rs1; Rsk2; S6K-alpha-3; p90-RSK 3; p90RSK3; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; RSK-2
• UniProt Entry Name: KS6A3_MOUSE

Uniprot Description

RSK2: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; RSK family; RSK subfamily

Cellular Component: cytoplasm; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; caspase inhibitor activity; transferase activity, transferring phosphorus-containing groups; magnesium ion binding; nucleotide binding; kinase activity; ribosomal protein S6 kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: regulation of translation; cellular response to stress; toll-like receptor signaling pathway; response to lipopolysaccharide; positive regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; cell cycle; phosphorylation; protein amino acid phosphorylation