Western Blot (WB)
Rabbit anti-Mouse Casein (kappa) Polyclonal Antibody | anti-Casein (kappa) antibody
Rabbit anti Casein kappa Polyclonal antibody
Reactivity
Mouse
Applications
Western Blot, ELISA, Immunoprecipitation
Purity
The Rabbit IgG is purified by Epitope Affinity Purification.
Synonyms
Casein (kappa); Polyclonal Antibody; Rabbit anti Casein kappa Polyclonal antibody; Rabbit anti-Csnk; Casein; Casein Kappa; Csnk; CSN10; CSN3.; anti-Casein (kappa) antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
This antibody recognizes ~21 kDa of mouse Casein protein. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile phosphate-buffered saline (pH7.2) containing antibody stabilizer
Applicable Applications for anti-Casein (kappa) antibody
Western Blot (WB), ELISA, Immunoprecipitation (IP)
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
IHC: 2-10 ug/ml
Flow cytometry: Not tested
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
IHC: 2-10 ug/ml
Flow cytometry: Not tested
Antigen Preparation
A synthetic peptide corresponding to the N-terminus of mouse Casein protein.
Positive Control
Kidney Tissue
Cellular Location
Cell Membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C.
The antibodies can be stored at 2-8°C for three months without detectable loss of activity.
Avoid repeated freezing-thawing cycles.
The antibodies can be stored at 2-8°C for three months without detectable loss of activity.
Avoid repeated freezing-thawing cycles.
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-Casein (kappa) antibody
Casein is the predominant phosphoprotein which has no disulphide bridges but rich in proline. It is found in fresh milk, as a salt of calcium. Casein is not coagulated by heat. It is precipitated by acids and by rennet, a proteolytic enzyme typically obtained from the stomachs of calves. The enzyme trypsin can hydrolyze off a phosphate-containing peptone. This protein is synthesized by mammary epithelium only in the presence of lactogenic hormone combination of insulin, hydrocortisone, and prolactin. Enhancement of the rate of secretion of newly-synthesised caseins occurs when mammary explants are challenged in vitro with agents that activate protein kinase A (PKA). The PKA-sensitive step (s) in casein secretion is early in the exocytosis pathway but inhibition of PKA does not impair casein maturation. PKA is located on membranes of vesicles situated in the Golgi region. Casein positive cells are present in all breast carcinomas with a variable distribution and degree of staining. Casein serves as an in vitro marker of terminally differentiated breast luminal epithelial cells.
References
P. Chandra Shekar et al. ?-Casein-deficient mice fail to lactate. PNAS 2006 May 23, 103 (21): 8000-8005
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Product Notes
The Casein (kappa) (Catalog #AAA462214) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Casein kappa Polyclonal antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Casein (kappa) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA, Immunoprecipitation (IP). Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml IHC: 2-10 ug/ml Flow cytometry: Not tested. Researchers should empirically determine the suitability of the Casein (kappa) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Casein (kappa), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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