Western Blot (WB)
Rabbit Actin, Smooth Muscle Antibody | anti-Actin, Smooth Muscle antibody
Rabbit anti SMA antibody (Smooth Muscle Actin)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA, Immunoprecipitation
Purity
The Rabbit IgG is purified by Epitope affinity purification
Synonyms
Actin; Smooth Muscle; Rabbit anti SMA antibody (Smooth Muscle Actin); Rabbit anti-Actin; ACTIN; SMA; Smooth muscle Actin; ACTG2; ACT; ACTA2; anti-Actin; Smooth Muscle antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes Actin protein. It cross-reacts to human, mouse, rat, chicken, bovine, etc.
Purity/Purification
The Rabbit IgG is purified by Epitope affinity purification
Form/Format
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.
Applicable Applications for anti-Actin, Smooth Muscle antibody
Western Blot (WB), Immunohistochemistry-paraffin (IHC-p), ELISA (EIA), Immunoprecipitation (IP)
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-10 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-10 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.
Antigen Preparation
A 10aa synthetic peptide corresponding to N-term of human Actin protein, smooth muscle specific. This sequence is identical in human, mouse, rat, bovine and chicken.
Positive Control
Kidney Tissue
Cellular Location
Cell Mmebrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2°C-8°C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Western Blot (WB)
Western Blot (WB)
Western Blot (WB)
(Western Blot: The whole lysate derived from Ht29 (20 ug/lane) immunoblotted by Rabbit anti –Actin (Cat# MBS462003) at 1:500. Observed a major immunoreactive band at molecular weight ~42 kDa.)
Western Blot (WB)
(Western Blot: The whole lysate derived from Ht29 (20 ug/lane) immunoblotted by Rabbit anti –Actin (Cat# MBS462003) at 1:500. Observed a major immunoreactive band at molecular weight ~42 kDa.)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Related Product Information for anti-Actin, Smooth Muscle antibody
The Actin protein a component of the eukaryotic cytoskeleton is highly conserved between multiple species. It is involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. The levels of the Actin protein may vary between different cell types. But are consistent within a given cell type, and for this reason, Actin protein levels are considered an internal control for protein loading.
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Product Notes
The Actin, Smooth Muscle (Catalog #AAA462003) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti SMA antibody (Smooth Muscle Actin) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Actin, Smooth Muscle can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-paraffin (IHC-p), ELISA (EIA), Immunoprecipitation (IP). Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 2-10 ug/ml Optimal dilutions should be determined by researchers for the specific applications. Researchers should empirically determine the suitability of the Actin, Smooth Muscle for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin, Smooth Muscle, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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