Testing Data
Rabbit anti-Drosophila Shaker Polyclonal Antibody
Rabbit anti Shaker
Reactivity
Drosophila
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The rabbit IgG is purified by Epitope Affinity Purification.
Synonyms
Shaker; Polyclonal Antibody; Rabbit anti Shaker; Rabbit anti Shaker Polyclonal antibody; Potassium voltage-gated channel protein shaker; CG12348-PE; Potassium channel protein; anti-Shaker antibody
Host
Rabbit
Reactivity
Drosophila
Clonality
Polyclonal
Specificity
This antibody recognizes ~73 kDa of Shaker protein.
Purity/Purification
The rabbit IgG is purified by Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile phosphate buffered saline (pH7.2) containing antibody stabilizer.
Applicable Applications for anti-Shaker antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
Suggested Dilution:
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
Optimal dilution should be determined by researchers for the specific applications.
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
Optimal dilution should be determined by researchers for the specific applications.
Antigen Preparation
A synthetic peptide derived from N-terminus of Shaker protein.
Positive Control
Shaker over-expressed HEK293
Cellular Localization
Membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-Shaker antibody
The assemblage of specific ion channels and receptors at synaptic sites is crucial for signaling between pre- and postsynaptic cells. DLG (Drosophila discs-large gene) is co-localized with Shaker K1 channels, which are clustered at glutamatergic synapses. DLG-Shaker interactions are required in vivo for shaker clustering at the neuromuscular junction. The channel protein Shaker which is properly synthesized, glycosylated, folded, assembled and delivered to the plasma membrane is very important for the potassium channel function.
References
• Francisco J. Tejedor, et al. Essential Role for dlg in Synaptic Clustering of Shaker K+ Channels In Vivo. The Journal of Neuroscience, January 1, 1997, 17(1):152-159.
Similar Products
Product Notes
The Shaker (Catalog #AAA462128) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Shaker reacts with Drosophila and may cross-react with other species as described in the data sheet. AAA Biotech's Shaker can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Suggested Dilution: Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 2-5 ug/ml Optimal dilution should be determined by researchers for the specific applications. Researchers should empirically determine the suitability of the Shaker for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Shaker, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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