Rabbit GSK-3b (pY216) Antibody | anti-GSK-3b (pY216) antibody

Rabbit anti Phospho- GSK-3beta (pTyr 216)

Cat. Num. AAA462202

• Reactivity: Human, Rat, Mouse, Bovine, Chicken
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Synonyms: GSK-3b (pY216); Rabbit anti Phospho- GSK-3beta (pTyr 216); Rabbit anti-Phospho-GSK3b (pY216); glycogen synthase kinase 3 beta (GSK3-b); anti-GSK-3b (pY216) antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Bovine, Chicken
• Specificity: This antibody recognizes GSK-3beta (pY216) with a phosphorylated site at Tyrosine 216. It does not crossreact with non-phosphospecific peptide.
• Purity/Purification: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer

• Applicable Applications for anti-GSK-3b (pY216) antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml; ; Optimal dilutions should be determined by researchers for the specific applications.
• Antigen Preparation: A synthetic peptide surrounding to the epitope -NVSYI- with a phosphorylation site at Tyr216 of human GSK3-beta protein. This sequence is identical among human, mouse, rat, bovine, dog and chicken species.
• Positive Control: 3T3 cell lysate
• Cellular Location: Cytoplasmic
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Testing Data

Related Product Information for anti-GSK-3b (pY216) antibody

Glycogen Synthase Kinase-3beta (GSK-3beta) is a serine/threonine kinase that affects glycogen metabolism by phosphorylating and down-regulating the activity of muscle glycogen synthase. Growth factor (such as Insulin) binds to its receptor, recruits PKB/AKT kinase to phosphorylate GSK-3beta on Serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. In contrast, the phosphorylation of tyrosine 216 in the catalytic domain increase this kinase activity to promote cell death under the absence of growth factors. GSK-3beta is also involved in Wnt signaling pathway where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein, thus the GSK-3beta inhibitor is considered as a potential approach in Alzheimer's Disease (AD) treatment.

References

Alan P. Kozikowski, et al. Highly Potent and Specific GSK-3beta Inhibitors That Block Tau Phosphorylation and Decrease alpha-Synuclein Protein Expression in a Cellular Model of Parkinson's Disease. ChemMedChem, 2006, 1 (2), 256-266.

NCBI and Uniprot Product Information

• NCBI GI #: 321461564
• Molecular Weight: 46 kDa
• NCBI Official Full Name: glycogen synthase kinase 3 beta