Western Blot
(Western Blot: The cell lysate of T293 with over-expressed recombinant protein GFP was resolved onto 10% SDS-PAGE, then transferred onto NC membrane. Followed by an immune-blotting with Rabbit anti- GFP (MBS462051) at 1:1000.)
Rabbit anti-Tag Antibody GFP Antibody | anti-GFP antibody
Rabbit anti GFP Polyclonal antibody
Reactivity
Tag Antibody
Applications
Western Blot, ELISA, Immunoprecipitation
Purity
The Rabbit IgG is purified by Affinity Chromatography.
Synonyms
GFP; Rabbit anti GFP Polyclonal antibody; Rabbit anti-GFP; Green Fluorescent Protein; anti-GFP antibody
Host
Rabbit
Reactivity
Tag Antibody
Isotype
IgG
Specificity
This antibody recognizes ~ 26 kDa of Green Fluorescent Protein. It is a tag antibody.
Purity/Purification
The Rabbit IgG is purified by Affinity Chromatography.
Form/Format
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.
Applicable Applications for anti-GFP antibody
Western Blot (WB), ELISA, Immunoprecipitation (IP)
Application Notes
Suggested Working Dilutions: WB: 0.1-1 ug/ml, ELISA: 0.01-0.1 ug/ml, IP: 2-5 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.
Optimal dilutions should be determined by researchers for the specific applications.
Antigen Preparation
A full length GFP recombinant protein.
Positive Control
Recombinant or native GFP.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2°C to 8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Western Blot
(Western Blot: The cell lysate of T293 with over-expressed recombinant protein GFP was resolved onto 10% SDS-PAGE, then transferred onto NC membrane. Followed by an immune-blotting with Rabbit anti- GFP (MBS462051) at 1:1000.)
Western Blot
(Western Blot: The cell lysate of T293 with over-expressed recombinant protein GFP was resolved onto 10% SDS-PAGE, then transferred onto NC membrane. Followed by an immune-blotting with Rabbit anti- GFP (MBS462051) at 1:1000.)
Related Product Information for anti-GFP antibody
The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
References
Prendergast F, Mann K (1978). "Chemical and physical properties of aequorin and the green fluorescent protein isolated from Aequorea forskålea". Biochemistry 17 (17): 3448-53
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
26.9 kDa
NCBI Official Full Name
GFP
Protein Family
Similar Products
Product Notes
The GFP (Catalog #AAA462051) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti GFP Polyclonal antibody reacts with Tag Antibody and may cross-react with other species as described in the data sheet. AAA Biotech's GFP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA, Immunoprecipitation (IP). Suggested Working Dilutions: WB: 0.1-1 ug/ml, ELISA: 0.01-0.1 ug/ml, IP: 2-5 ug/mlOptimal dilutions should be determined by researchers for the specific applications. Researchers should empirically determine the suitability of the GFP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFP, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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