Rabbit STAT4 Antibody | anti-STAT4 antibody

Rabbit anti STAT4 Polyclonal Antibody

Cat. Num. AAA460083

• Gene Names: STAT4; SLEB11
• Reactivity: Human, Rat, Mouse, Dog
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: The Rabbit IgG is purified by Epitope Affinity Purification.
• Synonyms: STAT4; Rabbit anti STAT4 Polyclonal Antibody; Signal transducer and activator of transcription 4 (STAT4); anti-STAT4 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Dog
• Specificity: This antibody recognizes STAT4 (pY693) with a phosphorylated sites of Tyr693. It does not cross-react with non-phosphospecific peptide.
• Purity/Purification: The Rabbit IgG is purified by Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer

• Applicable Applications for anti-STAT4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 0.1-1 ug/ml; ELISA 0.01-0.1 ug/ml; IP: 2-5 ug/ml; IHC: 1:100 ug/ml; Optimal dilutions should be determined by researchers for the specific applications.
• Preparation: A synthetic peptide surrounding to the C-terminus of STAT4 protein from human. This sequence is identical to mouse, rat, chicken, dog and bovine origins.
• Positive Control: Mouse kidney
• Nuclear Location: Nuclear
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at –20°C to –70°C. The antibodies can be stored at 2°C-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Western Blot (WB)

Related Product Information for anti-STAT4 antibody

STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor. Activation of STAT4 induces dimerization, nuclear translocation and DNA binding. STAT4 protein expression is restricted to the thymus, spleen and testis. Both tyrosine and serine residues on STAT4 become phosphorylated in response to IL-12 or IFN-alpha although serine phosphorylation is not required for DNA binding. STAT4 is activated in T-cells in response to the cytokine interleukin-12 (IL-12), and in NK cells by IL-2. STAT4-deficient mice display impaired IL-12 development of Th1 cells and enhanced development of Th2 cells.

NCBI and Uniprot Product Information

• NCBI GI #: 167832560
• NCBI GeneID: 6775
• Molecular Weight: 90 kDa
• NCBI Official Full Name: STAT4, partial
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 4
• NCBI Official Symbol: STAT4
• NCBI Official Synonym Symbols: SLEB11
• NCBI Protein Information: signal transducer and activator of transcription 4
• UniProt Protein Name: Signal transducer and activator of transcription 4
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT4

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

Uniprot Description

STAT4: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q32.2-q32.3

Molecular Function: protein binding

Biological Process: JAK-STAT cascade

Disease: Systemic Lupus Erythematosus, Susceptibility To, 11