Mouse anti-Human, Mouse IRAK4 (pT345) Monoclonal Antibody | anti-IRAK4 (pT345) antibody

Mouse anti Phospho-IRAK-4 (pThr345)

Cat. Num. AAA462225

• Gene Names: IRAK4; IPD1; REN64; NY-REN-64
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: The Mouse IgG is purified by Isotyping-specific Affinity Purification.
• Synonyms: IRAK4 (pT345); Monoclonal Antibody; Mouse anti Phospho-IRAK-4 (pThr345); Ms anti-Phospho-IRAK4 (pT345); Interleukin-1 receptor-associated kinase 4; IRAK-4; IPD1; REN64; NY-REN-64; anti-IRAK4 (pT345) antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: A8A8
• Specificity: This antibody recognizes IRAK-4 (pT345) with a phosphorylated site at Threonine 345. It does not crossreact with non-phosphospecific peptide.
• Purity/Purification: The Mouse IgG is purified by Isotyping-specific Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH 7.2) containing antibody stabilizer

• Applicable Applications for anti-IRAK4 (pT345) antibody: ELISA, Western Blot, Immunohistochemistry; Optimal dilutions should be determined by researchers for the specific applications.
• Application Notes: Western Blot:0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml; Immunohistochemistry: 2-5 ug/ml
• Antigen Preparation: A synthetic peptide surrounding to the epitope -VMTSR- with a phosphorylation site at Thr345 of human IRAK-4 protein. This sequence is identical among human, mouse, bovine and dog.
• Positive Control: Jurkat Cell lysate
• Cellular Location: Cytoplasmic
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2°C -8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Testing Data

Related Product Information for anti-IRAK4 (pT345) antibody

IRAK (IL-1 associated serine/threonine kinase) is a critical regulator of Interleukin-1 (IL-1) induced activation of the NFkappaB pathway. IL-1 stimulation leads to the recruitment of interleukin-1 receptor-associated kinase (IRAK) to the IL-1 receptor, where IRAK is phosphorylated, ubiquitinated, and eventually degraded. There are four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). IRAK-4, recently found another IRAK family member necessary for the IL-1 pathway, is able to phosphorylate IRAK in vitro suggests that IRAK-4 might be the IRAK kinase. IRAK4 is required for the efficient recruitment of IRAK to the IL-1 receptor complex.

References

Li S et al (2002) IRAK-4, A novel member of the IRAK family with the properties of an IRAK-kinase. PNAS 99 (8): 5567-5572.

NCBI and Uniprot Product Information

• NCBI GI #: 166795295
• NCBI GeneID: 51135
• NCBI Accession #: NP_001107654.1
• NCBI GenBank Nucleotide #: NM_001114182.2
• UniProt Accession #: Q69FE3; Q7Z6A7; Q7Z6A8; Q9NWZ3
• Molecular Weight: ~51 kDa
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 4 isoform a
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 4
• NCBI Official Symbol: IRAK4
• NCBI Official Synonym Symbols: IPD1; REN64; NY-REN-64
• NCBI Protein Information: interleukin-1 receptor-associated kinase 4; IRAK-4; renal carcinoma antigen NY-REN-64
• UniProt Protein Name: Interleukin-1 receptor associated kinase-4
• UniProt Gene Name: IRAK4
• UniProt Entry Name: Q69FE3_HUMAN

NCBI Description

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [supplied by RefSeq]

Uniprot Description

IRAK4: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; IRAK family

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: extracellular space; cytoplasm; plasma membrane; endosome membrane; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; interleukin-1 receptor binding; magnesium ion binding; ATP binding; protein kinase activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; cytokine and chemokine mediated signaling pathway; positive regulation of smooth muscle cell proliferation; cytokine production; protein amino acid phosphorylation; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor signaling pathway; JNK cascade; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway

Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 1; Irak4 Deficiency