Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed BrdU-labeled HeLa cells showing nuclear localization.)
Mouse BrdU Monoclonal Antibody | anti-BrdU antibody
BrdU (Bu20a) Antibody
Reactivity
Bromodeoxyuridine
Applications
Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
BrdU; Monoclonal Antibody; BrdU (Bu20a) Antibody; Bromodeoxyuridine antibody; BUdr antibody; anti-BrdU antibody
Host
Mouse
Reactivity
Bromodeoxyuridine
Clonality
Monoclonal
Clone Number
1G1
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-BrdU antibody
Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS)
Application Notes
IHC: 1:50-500
FC/FACS: 1ug per 1 x 106 cells
FC/FACS: 1ug per 1 x 106 cells
Immunogen
Peptide
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed BrdU-labeled HeLa cells showing nuclear localization.)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed BrdU-labeled HeLa cells showing nuclear localization.)
Testing Data
(Intracellular FCM analysis of fixed and permeabilized untreated (dotted blue histogram) and BrdU treated (solid orange histogram) K-562 cells. Green line histogram represents the isotype control, normal mouse IgG1.)
Testing Data
(Intracellular FCM analysis of fixed and permeabilized untreated (dotted blue histogram) and BrdU treated (solid orange histogram) K-562 cells. Green line histogram represents the isotype control, normal mouse IgG1.)
Related Product Information for anti-BrdU antibody
The halogenated pyrimidine thymidine analog bromodeo-xyuridine (BrdU) is incorporated into newly synthesized DNA strands of S-phase cells and is useful for estimating the fraction of cells in S-phase. Additionally, the analysis of the uptake of BrdU is a reliable method to quantitate the degree of DNA-synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. Anti-BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with crosslinking agents such as formalin but may decrease the specificity of BrdUrd immunodetection. The monoclonal antibody Bu20a against BrdU stains BrdU incorporated into the nulcei of a wide range of proliferating cell types including human tumors growing in nude mice and tonsil lymphoid.
Product Categories/Family for anti-BrdU antibody
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Product Notes
The BrdU (Catalog #AAA9435145) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BrdU (Bu20a) Antibody reacts with Bromodeoxyuridine and may cross-react with other species as described in the data sheet. AAA Biotech's BrdU can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS). IHC: 1:50-500 FC/FACS: 1ug per 1 x 106 cells. Researchers should empirically determine the suitability of the BrdU for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BrdU, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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