Agrin Recombinant Protein | AGRN recombinant protein

Recombinant Agrin (AGRN)

Cat. Num. AAA2034412

• Gene Names: AGRN; HSPG
• Reactivity: Human
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Agrin; Recombinant Agrin (AGRN); Agrin Proteoglycan; AGRN recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: >90%
• Sequence Length: 1955

• Applicable Applications for AGRN recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Fragment: Pro1329~Cys1548
• Tag: Two N-terminal Tags, His-tag and T7-tag
• Expression System: Prokaryotic Expression
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 211121
• NCBI GeneID: 396538
• Molecular Weight: 26.8kDa
• NCBI Official Full Name: agrin
• NCBI Official Synonym Full Names: agrin
• NCBI Official Symbol: AGRN
• NCBI Official Synonym Symbols: HSPG
• NCBI Protein Information: agrin
• UniProt Protein Name: Agrin
• Protein Family: Agrin
• UniProt Gene Name: AGRN
• UniProt Synonym Gene Names: AGRIN; C90; C22
• UniProt Entry Name: AGRIN_CHICK

Uniprot Description

Isoform 1: heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. Component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses.Isoform 9: transmembrane agrin (TM-agrin), the predominant form in neurons of the brain, induces dendritic filopodia and synapse formation in mature hippocampal neurons in large part due to the attached glycosaminoglycan chains and the action of Rho-family GTPases.Isoform 2, isoform 4 and isoform 7: muscle agrin isoforms, which lack the 8-amino acid insert at the 'B' site, but with the insert at the'A' site have no AChr clustering activity nor MUSK activation but bind heparin. Bind alpha-dystroglycan with lower affinity.Isoform 5: muscle agrin A0B0 lacking inserts at both 'A' and 'B' sites has no heparin-binding nor AChR clustering activity but binds strongly alpha-dystroglycan.Agrin N-terminal 110 kDa subunit: is involved in modulation of growth factor signaling (). Involved also in the regulation of neurite outgrowth probably due to the presence of the glycosaminoglcan (GAG) side chains of heparan and chondroitin sulfate attached to the Ser/Thr- and Gly/Ser-rich regions. Also involved in modulation of growth factor signaling.