Western Blot (WB)
(Sample Data: Representative Western blot with Anti-AFX (1:500) using 3 ug, 10 ug and 30 ug of 3T6 cell lysate.)
Rabbit AFX Polyclonal Antibody | anti-AFX antibody
Anti-AFX
Reactivity
Western blot human, mouse AFXAFX from other species may also be detectable.
Applications
Western Blot
Purity
Affinity chromatography
Synonyms
AFX; Polyclonal Antibody; Anti-AFX; anti-AFX antibody
Host
Rabbit
Reactivity
Western blot human, mouse AFX
AFX from other species may also be detectable.
AFX from other species may also be detectable.
Clonality
Polyclonal
Specificity
Recognizes the AFX protein
Purity/Purification
Affinity chromatography
Form/Format
PBS, pH7.4, 0.02%NaN3, 50% glycerol
Applicable Applications for anti-AFX antibody
Western Blot (WB)
Application Notes
WB: 21560:22059
Immunogen
Synthetic peptide corresponding to amino acids 195-199 of human AFX
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Representative Western blot with Anti-AFX (1:500) using 3 ug, 10 ug and 30 ug of 3T6 cell lysate.)
Western Blot (WB)
(Sample Data: Representative Western blot with Anti-AFX (1:500) using 3 ug, 10 ug and 30 ug of 3T6 cell lysate.)
Related Product Information for anti-AFX antibody
AFX is a member of the O class of winged helix/forkhead transcription factor family. AFX proteins are involved in cell cycle regulation and inactivation of these proteins help in oncogenic transformation. AFX regulates apoptosis in part by suppressing the level of antiapoptotic BCLX through the transcriptional repressor BCL6 (1). AFX is down-regulated in patients with inflammatory bowel disease. AFX is expressed in human luteinized mural granulosa cells and may be involved in human folliculogenesis and luteinization. AFX acts as a novel anticancer agent for HER2-overexpressing cells.
Product Categories/Family for anti-AFX antibody
References
1. Tang, T. et al: The forkhead transcription factor AFX activates apoptosis by induction of the BCL-6 transcriptional repressor. J. Biol. Chem. 27714255-14265, 2002.
Similar Products
Product Notes
The AFX (Catalog #AAA515836) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-AFX reacts with Western blot human, mouse AFX AFX from other species may also be detectable. and may cross-react with other species as described in the data sheet. AAA Biotech's AFX can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 21560:22059. Researchers should empirically determine the suitability of the AFX for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AFX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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