Rabbit EGFR Polyclonal Antibody | anti-EGFR antibody

Anti-EGFR

Cat. Num. AAA515940

• Reactivity: Western blot EGFR from human cells; EGFR from other species may also be detectable.
• Applications: Western Blot
• Purity: Affinity chromatography
• Synonyms: EGFR; Polyclonal Antibody; Anti-EGFR; anti-EGFR antibody

• Host: Rabbit
• Reactivity: Western blot EGFR from human cells; EGFR from other species may also be detectable.
• Clonality: Polyclonal
• Specificity: Recognizes the EGFR protein
• Purity/Purification: Affinity chromatography
• Form/Format: PBS pH7.4, 0.02% sodium azide, 50% glycerol

• Applicable Applications for anti-EGFR antibody: Western Blot (WB)
• Application Notes: WB: 20430:21429
• Immunogen: Synthetic peptide corresponding to amino acids 1195-1199 of human EGFR (protein accession number P00533)
• Preparation and Storage: Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Western Blot (WB)

(Sample Data: Representative western blot with Anti-EGFR (1:1000) using 3 ug, 10 ug and 30 ug of HeLa cell lysate.)

Related Product Information for anti-EGFR antibody

EEGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival (1). Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms (2). Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation.

Product Categories/Family for anti-EGFR antibody

Primary Antibodies; AKT/PKB Pathway; Angiogenesis; Apoptosis/Autophagy; Cancer; Cardiovascular Disease; Cell Cycle; ERK/MAPK Pathway; Inflammation; Invasion/Metastasis; Metabolic Disorder; PKA/PKC Pathway; Receptor Tyrosine Kinases

References

1. Wang K, et al: Epidermal growth factor receptor-deficient mice have delayed primary endochondral ossification because of defective osteoclast recruitment. J. Biol. Chem. 279: 53848-53856, 2004.
2. Kobayashi S, et al: EGFR mutation and resistance of non-small-cell lung cancer to gefitinib. New Eng. J. Med. 352: 786-792, 2005.