Western Blot (WB)
(Sample Data: Representative western blot with Anti-SMC1 (1:1000) using 3 ug, 10 ug and 30 ug of HeLa cell lysate.)
Rabbit SMC1 Polyclonal Antibody | anti-SMC1 antibody
Anti-SMC1
Reactivity
Western blot SMC1 from human, mouse cellsSMC1 from other species may also be detectable.
Applications
Western Blot
Purity
Affinity chromatography
Synonyms
SMC1; Polyclonal Antibody; Anti-SMC1; anti-SMC1 antibody
Host
Rabbit
Reactivity
Western blot SMC1 from human, mouse cells
SMC1 from other species may also be detectable.
SMC1 from other species may also be detectable.
Clonality
Polyclonal
Specificity
Recognizes the SMC1 protein
Purity/Purification
Affinity chromatography
Form/Format
PBS pH7.4, 0.02% sodium azide, 50% glycerol
Applicable Applications for anti-SMC1 antibody
Western Blot (WB)
Application Notes
WB: 18463:19462 dilution
Immunogen
Synthetic peptide corresponding to amino acids 955-959 of human SMC1 (Q14683)
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Representative western blot with Anti-SMC1 (1:1000) using 3 ug, 10 ug and 30 ug of HeLa cell lysate.)
Western Blot (WB)
(Sample Data: Representative western blot with Anti-SMC1 (1:1000) using 3 ug, 10 ug and 30 ug of HeLa cell lysate.)
Related Product Information for anti-SMC1 antibody
SMC1 is a member of the structural maintenance of chromosomes 1A family that is localized at the kinetochores and is involved in cell division. SMC1is part of the cohesin multiprotein complex which is required for sister chromatid cohesion (1). Most of the cohesin complexes dissociate from the chromosomes before mitosis but remain at the kinetochore and so SMC1 is an important part of the functional kinetochores. SMC1 protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair (2).
Product Categories/Family for anti-SMC1 antibody
References
1. Gruber, S. et al: Chromosomal cohesin forms a ring. Cell 112: 765-777, 2003.
2. Revenkova, E. et al: Cornelia de Lange syndrome mutations in SMC1A or SMC3 affect binding to DNA. Hum. Molec. Genet. 18: 418-427, 2009.
2. Revenkova, E. et al: Cornelia de Lange syndrome mutations in SMC1A or SMC3 affect binding to DNA. Hum. Molec. Genet. 18: 418-427, 2009.
Similar Products
Product Notes
The SMC1 (Catalog #AAA516216) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SMC1 reacts with Western blot SMC1 from human, mouse cells SMC1 from other species may also be detectable. and may cross-react with other species as described in the data sheet. AAA Biotech's SMC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 18463:19462 dilution. Researchers should empirically determine the suitability of the SMC1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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