Western Blot (WB)
(Sample Data: Representative western blot with Anti-PKCzeta (1:1000) using 3 ug, 10 ug, and 30 ug of 3T6 cell lysate.)
Rabbit PKC zeta Polyclonal Antibody | anti-PKCzeta antibody
Anti-PKC zeta
Reactivity
Western blot of human PKCzPKCz from other species may also be detectable
Applications
Western Blot
Purity
Affinity chromatography
Synonyms
PKC zeta; Polyclonal Antibody; Anti-PKC zeta; anti-PKCzeta antibody
Host
Rabbit
Reactivity
Western blot of human PKCz
PKCz from other species may also be detectable
PKCz from other species may also be detectable
Clonality
Polyclonal
Specificity
Recognizes the PKCzeta protein
Purity/Purification
Affinity chromatography
Form/Format
PBS pH7.4, 0.02% sodium azide, 50% glycerol
Applicable Applications for anti-PKCzeta antibody
Western Blot (WB)
Application Notes
WB: 19027:20026
Immunogen
Synthetic peptide corresponding to amino acids 408-412 (TSTFC) of human PKCzeta
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Representative western blot with Anti-PKCzeta (1:1000) using 3 ug, 10 ug, and 30 ug of 3T6 cell lysate.)
Western Blot (WB)
(Sample Data: Representative western blot with Anti-PKCzeta (1:1000) using 3 ug, 10 ug, and 30 ug of 3T6 cell lysate.)
Related Product Information for anti-PKCzeta antibody
PKCzeta is an atypical isoform of the PKC family. PKCzeta is found in both particulate and soluble fractions and cannot be activated by phorbol ester. Overexpression of PKCzeta and subsequent phorbol ester treatment abolished phorbol ester-induced reduction in cell proliferation (1). Overexpression of PKCzeta also potentiates phorbol ester-induced mitogen-activated protein (MAP) kinase activation in a PKC-dependent manner. PKCzeta is an upstream modulator of p70S6K, an important regulator of cell proliferation (2).
Product Categories/Family for anti-PKCzeta antibody
References
1. Kim, SJ. Et al: Phorbol ester effects in atypical protein kinase C zeta overexpressing NIH3T3 cells: possible evidence for crosstalk between protein kinase C isoforms. Biochem Biophys Res Commun. 1997 Aug 18;237(2):336-9.
2. Romanelli, A. et al: p70 S6 kinase is regulated by protein kinase Czeta and participates in a phosphoinositide 3-kinase-regulated signalling complex. Mol Cell Biol. 1999 Apr;19(4):2921-8.
2. Romanelli, A. et al: p70 S6 kinase is regulated by protein kinase Czeta and participates in a phosphoinositide 3-kinase-regulated signalling complex. Mol Cell Biol. 1999 Apr;19(4):2921-8.
Similar Products
Product Notes
The PKCzeta (Catalog #AAA516163) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PKC zeta reacts with Western blot of human PKCz PKCz from other species may also be detectable and may cross-react with other species as described in the data sheet. AAA Biotech's PKC zeta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 19027:20026. Researchers should empirically determine the suitability of the PKCzeta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKC zeta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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