Immunoprecipitation (IP)
(Sample Data: Representative immunoprecipitation (left image) with 2 ug of Anti- PKCu using 500 ug of HEK-293T cell lysate. Representative western blot (right image) with Anti- PKCu (1:1000) using 3 ug, 10 ug, and 30 ug of HEK-293T cell lysate.)
Rabbit PKC mu Polyclonal Antibody | anti-PKCmu antibody
Anti-PKC mu
Reactivity
Western blot human PKCmuPKCmu from other species may also be detectable.
Applications
Western Blot
Purity
Affinity chromatography
Synonyms
PKC mu; Polyclonal Antibody; Anti-PKC mu; anti-PKCmu antibody
Host
Rabbit
Reactivity
Western blot human PKCmu
PKCmu from other species may also be detectable.
PKCmu from other species may also be detectable.
Clonality
Polyclonal
Specificity
Recognizes the PKCmu protein
Purity/Purification
Affinity chromatography
Form/Format
PBS pH7.4, 0.02% sodium azide, 50% glycerol
Applicable Applications for anti-PKCmu antibody
Western Blot (WB)
Application Notes
WB: 21867:22866
Immunogen
Synthetic peptide corresponding to amino acids 736-740 of human PKCmu
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Immunoprecipitation (IP)
(Sample Data: Representative immunoprecipitation (left image) with 2 ug of Anti- PKCu using 500 ug of HEK-293T cell lysate. Representative western blot (right image) with Anti- PKCu (1:1000) using 3 ug, 10 ug, and 30 ug of HEK-293T cell lysate.)
Immunoprecipitation (IP)
(Sample Data: Representative immunoprecipitation (left image) with 2 ug of Anti- PKCu using 500 ug of HEK-293T cell lysate. Representative western blot (right image) with Anti- PKCu (1:1000) using 3 ug, 10 ug, and 30 ug of HEK-293T cell lysate.)
Western Blot (WB)
(Sample Data: Representative western blot with Anti- PKCu (1:1000) using 20ng of GST-tagged human recombinant PKCalpha, PKCbetaI, PKCbetaII, PKCgamma, PKCdelta, PKCepsilon, PKCeta, PKCtheta, PKCu, PKCNu, PKCzeta, and PKCiota.)
Western Blot (WB)
(Sample Data: Representative western blot with Anti- PKCu (1:1000) using 20ng of GST-tagged human recombinant PKCalpha, PKCbetaI, PKCbetaII, PKCgamma, PKCdelta, PKCepsilon, PKCeta, PKCtheta, PKCu, PKCNu, PKCzeta, and PKCiota.)
Related Product Information for anti-PKCmu antibody
Protein kinase C mu (PKCmu) is a member of the protein kinase C (PKC) family that differs from the other PKC isoenzymes in structural and enzymatic properties. PKCmu is a ubiquitous in nature with the highest expression in the thymus, lung and peripheral blood mononuclear cells (1). PKCmu forms a complex in vivo with a phosphatidylinositol 4-kinase and a phosphatidylinositol-4-phosphate 5-kinase. A region of PKCmu between the amino-terminal transmembrane domain and the pleckstrin homology domain is shown to be involved in the association with the lipid kinases (2).
Product Categories/Family for anti-PKCmu antibody
References
1. Rennecke, J. et al: Immunological demonstration of protein kinase C mu in murine tissues and various cell lines. Eur J Biochem. 1996 Dec 1;242(2):428-32.
2. Nishikawa, K. et al: Association of protein kinase Cmu with type II phosphatidylinositol 4-kinase and type phosphatidylinositol-4-phosphate 5-kinase. J Biol Chem. 1998 Sep 4;273(36):23126-33.
2. Nishikawa, K. et al: Association of protein kinase Cmu with type II phosphatidylinositol 4-kinase and type phosphatidylinositol-4-phosphate 5-kinase. J Biol Chem. 1998 Sep 4;273(36):23126-33.
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Product Notes
The PKCmu (Catalog #AAA516161) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PKC mu reacts with Western blot human PKCmu PKCmu from other species may also be detectable. and may cross-react with other species as described in the data sheet. AAA Biotech's PKC mu can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 21867:22866. Researchers should empirically determine the suitability of the PKCmu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKC mu, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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