Rabbit NEDD4 Polyclonal Antibody | anti-NEDD4 antibody

Phospho-NEDD4 (Ser670) Antibody

Cat. Num. AAA9613137

• Gene Names: NEDD4; RPF1; NEDD4-1
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (92%), Sheep (100%), Rabbit (100%), Dog (100%)
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: NEDD4; Polyclonal Antibody; Phospho-NEDD4 (Ser670) Antibody; Cell proliferation-inducing gene 53 protein; E3 ubiquitin protein ligase Nedd4; E3 ubiquitin-protein ligase NEDD4; HECT-type E3 ubiquitin transferase NEDD4; KIAA0093; NEDD 4; NEDD-4; Nedd4; NEDD4_HUMAN; Neural precursor cell expressed developmentally down regulated 4; Neural precursor cell expressed developmentally down-regulated protein 4; neural precursor cell expressed; developmentally down-regulated 4; E3 ubiquitin protein ligase; PIG53; receptor-potentiating factor 1; RPF1; anti-NEDD4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (92%), Sheep (100%), Rabbit (100%), Dog (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-NEDD4 (Ser670) Antibody detects endogenous levels of NEDD4 only when phosphorylated at Ser670.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-NEDD4 antibody: Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human NEDD4 around the phosphorylation site of Ser670.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Auto-ubiquitinated.
• Subunit Structure: Interacts with UBE2D2. Binds SCNN1A, SCNN1B and SCNN1G. Binds, in vitro, through the WW2 and WW3 domains, to neural isoforms of ENAH that contain the PPSY motif. Interacts with BEAN1, LITAF, RNF11, WBP1, WBP2, PMEPAI and PRRG2 (By similarity). Interacts with NDFIP1 and NDFIP2; this interaction activates the E3 ubiquitin-protein ligase and may induce its recruitment to exosomes (By similarity). Interaction with PTEN is questionable according to. Interacts (via C2 domain) with GRB10 (via SH2 domain). Interacts with ERBB4 (By similarity). Interacts with TNIK; the interaction is direct, allows the TNIK-dependent recruitment of RAP2A and its ubiquitination by NEDD4. Interacts (via WW3 domain) with TNK2; EGF promotes this interaction. Interacts (via WW3 domain) with FGFR1 (via C-terminus). Interacts with OTUD7B. Interacts with ISG15. Interacts (via WW domain) with RAPGEF2; this interaction leads to ubiquitination and degradation via the proteasome pathway. Interacts (via WW domains) with ARRDC3 (via PPXY motifs). Interacts with LAPTM4B; may play a role in the lysosomal sorting of LAPTM4B. Interacts (via WW domains) with ARRDC1, ARRDC2 and ARRDC3. Interacts with ZBTB7B (By similarity). (Microbial infection) Interacts with viral proteins that contain a late-budding motif P-P-P-Y. This interaction is essential for viral particle budding of many retroviruses, like HTLV-1 Gag and MLV Gag. Interacts with Herpes simplex virus 2 (HHV-2) protein UL56; this interaction induces NEDD4 degradation. Interacts with Ebola virus protein VP40.
• Similarity: The WW domains mediate interaction with PPxY motif-containing proteins (PubMed:21191027). The WW domains mediate interaction with LITAF, RNF11, WBP1, WBP2, PMEPAI, NDFIP1 and PRRG2 (By similarity).
• Subcellular Location: Cytoplasm. Cell membrane>Peripheral membrane protein.; Note: Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity). May be recruited to exosomes by NDFIP1.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from various samples, using Phospho-NEDD4(Ser670) Antibody.Lane 1: B16F10 cells, blocked with antigen-specific peptides,Lane 2: B16F10 cells,Lane 3: C6 cells.)

Western Blot (WB)

(Western blot analysis of extracts from SK-MEL-28, using Phospho-NEDD4(Ser670) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Immunohistochemistry (IHC)

(At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(At 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Related Product Information for anti-NEDD4 antibody

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. According tothe direct link between NEDD4 and PTEN regulation through polyubiquitination described inis questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1. (Microbial infection) Involved in the ubiquitination of Ebola virus protein VP40 which plays a role in viral budding.

NCBI and Uniprot Product Information

• NCBI GI #: 313104311
• NCBI GeneID: 4734
• UniProt Accession #: P46934; A1KY35; A6ND72; A7MD29; B4E2R7; B7ZM59; B7ZM60; B9EGN5; D6RF89
• Molecular Weight: 1319
• NCBI Official Full Name: E3 ubiquitin-protein ligase NEDD4
• NCBI Official Synonym Full Names: neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
• NCBI Official Symbol: NEDD4
• NCBI Official Synonym Symbols: RPF1; NEDD4-1
• NCBI Protein Information: E3 ubiquitin-protein ligase NEDD4; receptor-potentiating factor 1; cell proliferation-inducing gene 53 protein; neural precursor cell expressed developmentally down-regulated protein 4
• UniProt Protein Name: E3 ubiquitin-protein ligase NEDD4
• Protein Family: Nedd4 binding protein
• UniProt Gene Name: NEDD4
• UniProt Synonym Gene Names: KIAA0093; NEDD4-1; NEDD-4
• UniProt Entry Name: NEDD4_HUMAN

Uniprot Description

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins (PubMed:23644597). Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046).