SDS-Page
(AbFlex c-Myc antibody (rAb) tested by Western Blot. HEK293 cells were transiently transfected with empty vector (Lane 1) or with human c-Myc protein (Lane 2). 10 ug HEK293 whole cell extract was loaded on SDS-PAGE gel and probed with AbFlex c-Myc antibody at 1 ug/ml. MW: 68 kDa.)
Mouse anti-Human c-Myc Antibody | anti-c-Myc antibody
AbFlex c-Myc antibody (rAb)
WB: 1ug/ml dilution AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
SDS-Page
(AbFlex c-Myc antibody (rAb) tested by Western Blot. HEK293 cells were transiently transfected with empty vector (Lane 1) or with human c-Myc protein (Lane 2). 10 ug HEK293 whole cell extract was loaded on SDS-PAGE gel and probed with AbFlex c-Myc antibody at 1 ug/ml. MW: 68 kDa.)
SDS-Page
(AbFlex c-Myc antibody (rAb) tested by Western Blot. HEK293 cells were transiently transfected with empty vector (Lane 1) or with human c-Myc protein (Lane 2). 10 ug HEK293 whole cell extract was loaded on SDS-PAGE gel and probed with AbFlex c-Myc antibody at 1 ug/ml. MW: 68 kDa.)
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. c-Myc is a sequence specific DNA binding transcription factor that regulates gene expression, especially genes involved in cell division. Myc family members contain a basic Helix-Loop-Helix leucine zipper (bHLHZ) domain. Myc family proteins associated with thousands of genes, especially those involved in cell growth and proliferation. Myc serves as an integrator of signal transduction, linking extracellular signals to an acute cellular growth response. Mutations and overexpression of c-Myc are associated with cell proliferation and cancer.
NCBI and Uniprot Product Information
NCBI Description
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]
Uniprot Description
Myc: a proto-oncogenic transcription factor that plays a role in cell proliferation, apoptosis and in the development of human tumors. Seems to activate the transcription of growth-related genes.
Protein type: DNA-binding; Transcription factor; Oncoprotein; Nucleolus
Chromosomal Location of Human Ortholog: 8q24.21
Cellular Component: nucleoplasm; protein complex; nucleolus; nucleus; cytosol
Molecular Function: protein dimerization activity; protein binding; DNA binding; protein complex binding; transcription factor activity; transcription factor binding
Biological Process: oxygen transport; cellular iron ion homeostasis; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; chromosome organization and biogenesis; positive regulation of fibroblast proliferation; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to gamma radiation; cell cycle arrest; response to drug; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; regulation of telomere maintenance; MAPKKK cascade; negative regulation of cell division; negative regulation of stress-activated MAPK cascade; negative regulation of monocyte differentiation; chromatin remodeling; ureteric bud branching; negative regulation of fibroblast proliferation; regulation of gene expression; energy reserve metabolic process; positive regulation of transcription from RNA polymerase II promoter; gene expression; response to DNA damage stimulus; positive regulation of epithelial cell proliferation; negative regulation of apoptosis
Disease: Burkitt Lymphoma
Research Articles on c-Myc
Similar Products
Product Notes
The c-Myc myc (Catalog #AAA389470) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex c-Myc antibody (rAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's c-Myc can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1ug/ml dilution AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone. Researchers should empirically determine the suitability of the c-Myc myc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Myc, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
