Typical Testing Data/Standard Curve (for reference only)
Bovine Phosphoenolpyruvate carboxykinase, cytosolic [GTP] ELISA Kit | PPCK1 elisa kit
Bovine Phosphoenolpyruvate carboxykinase, cytosolic [GTP] ELISA Kit
Gene Names
PCK1; PPCK1
Reactivity
Bovine
Synonyms
Phosphoenolpyruvate carboxykinase; cytosolic [GTP]; Bovine Phosphoenolpyruvate carboxykinase; cytosolic [GTP] ELISA Kit; PPCK1 elisa kit
Reactivity
Bovine
Specificity
This assay recognizes recombinant and natural bovine PEPCK-C. No significant cross-reactivity or interference was observed.
Sequence Length
622
Samples
Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Detection Range
0.31 ng/ml -20.0 ng/ml.
Sensitivity
The minimum detectable dose of bovine PEPCK-C is typically less than 0.15 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.
Intended Uses
This immunoassay kit allows for the in vitro quantitative determination of bovine PEPCK-C concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PPCK1 elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PEPCK-C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PEPCK-C and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PEPCK-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PEPCK-C in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
69,353 Da
NCBI Official Full Name
phosphoenolpyruvate carboxykinase, cytosolic
NCBI Official Symbol
PCK1
NCBI Official Synonym Symbols
PPCK1
NCBI Protein Information
phosphoenolpyruvate carboxykinase, cytosolic [GTP]
UniProt Protein Name
Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Protein Family
UniProt Gene Name
PCK1
UniProt Synonym Gene Names
PEPCK-C
UniProt Entry Name
PCKGC_BOVIN
Uniprot Description
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Research Articles on PPCK1
Similar Products
Product Notes
The Bovine PPCK1 pck1 (Catalog #AAA288548) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA288548 ELISA Kit recognizes Bovine PPCK1. It is sometimes possible for the material contained within the vial of "Phosphoenolpyruvate carboxykinase, cytosolic [GTP], ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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