Mouse VEGFB ELISA Kit | VEGFB elisa kit

Mouse VEGFB ELISA Kit

Cat. Num. AAA8248466

• Gene Names: Vegfb; Vrf; VEGF-B
• Reactivity: Mouse
• Synonyms: VEGFB; Mouse VEGFB ELISA Kit; VRF; Vascular endothelial growth factor B; VEGF-B; VEGF-related factor; VEGFB elisa kit

• Reactivity: Mouse
• Specificity: The Mouse VEGFB ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse VEGFB proteins within the range of 31.2 pg/ml - 2000 pg/ml.
• Sequence Length: 188

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <2 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VEGFB elisa kit

Principle of the Assay: The Mouse VEGFB ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse VEGFB in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse VEGFB coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGFB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse VEGFB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGFB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. And it is important for the protection of neurons in the retina and thecerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.

NCBI and Uniprot Product Information

• NCBI GI #: 297632418
• NCBI GeneID: 22340
• NCBI Accession #: NP_001172093.1
• NCBI GenBank Nucleotide #: NM_001185164.1
• UniProt Accession #: P49766; Q3UG04; Q5D0B1; Q64290
• Molecular Weight: 21,442 Da
• NCBI Official Full Name: vascular endothelial growth factor B isoform Vegf-b167
• NCBI Official Synonym Full Names: vascular endothelial growth factor B
• NCBI Official Symbol: Vegfb
• NCBI Official Synonym Symbols: Vrf; VEGF-B
• NCBI Protein Information: vascular endothelial growth factor B
• UniProt Protein Name: Vascular endothelial growth factor B
• Protein Family: Vascular endothelial growth factor
• UniProt Gene Name: Vegfb
• UniProt Synonym Gene Names: Vrf; VEGF-B; VRF
• UniProt Entry Name: VEGFB_MOUSE

Uniprot Description

VEGFB: Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. Homodimer; disulfide-linked. Can also form heterodimer with VEGF. Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Cellular Component: extracellular region; extracellular space; intracellular

Molecular Function: growth factor activity; protein heterodimerization activity; protein homodimerization activity; vascular endothelial growth factor receptor 1 binding

Biological Process: angiogenesis; cardiac muscle contraction; heart development; induction of positive chemotaxis; positive regulation of angiogenesis; positive regulation of endothelial cell proliferation; protein amino acid O-linked glycosylation; response to hypoxia; vascular endothelial growth factor receptor signaling pathway