LIF recombinant protein

LIF Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8118864

• Purity: >85 % as determined by SDS-PAGE
• Synonyms: LIF; LIF Protein; Mouse; Recombinant (His Tag); Mouse LIF Protein (His Tag); leukemia inhibitory factor; LIF recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 191aa
• Sequence: Met1-Phe203

• Species: Mouse
• Tag: C-His
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser24
• Protein Construction: |A DNA sequence encoding the extracellular domain of mouse LIF (P09056) (Met 1-Phe 203) was fused with a polyhistidine tag at the C-terminus
• Activity: Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.1-0.5ng/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Testing Data

(Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.1-0.5 ng/ml.)

Related Product Information for LIF recombinant protein

Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It is involved in growth promotion and cell differentiation of different types of target cells, influence bone metabolism, cachexia, neural development, embryogenesis, and inflammation. LIF has potent proinflammatory properties, being the inducer of the acute phase protein synthesis and affecting cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic, and neuronal cells. LIF binds to the specific LIF receptor (LIFR-alpha) which forms a heterodimer with a specific subunit common to all members of that family of receptors, the GP130 signal-transducing subunit. This leads to the activation of the JAK/STAT and MAPK cascades. Due to its polyfunctional activities, LIF is involved in the pathogenic events and development of many diseases of various origins.

References

Salas EM, et al. (2011) LIF, a Novel STAT5-Regulated Gene, Is Aberrantly Expressed in Myeloproliferative Neoplasms. Genes Cancer. 2 (5): 593-6.Chodorowska G, et al. (2004) Leukemia inhibitory factor (LIF) and its biological activity. Ann Univ Mariae Curie Sklodowska Med. 59 (2): 189-93.Garcia-Campana AM, et al. (2007) LIF detection of peptides and proteins in CE. Electrophoresis. 28 (1-2): 208-32.

NCBI and Uniprot Product Information

• NCBI GI #: 88014736
• NCBI GeneID: 16878
• NCBI Accession #: NP_001034626.1
• NCBI GenBank Nucleotide #: NM_001039537.2
• UniProt Accession #: P09056
• Molecular Weight: 22,287 Da
• NCBI Official Full Name: leukemia inhibitory factor isoform b
• NCBI Official Synonym Full Names: leukemia inhibitory factor
• NCBI Official Symbol: Lif
• NCBI Protein Information: leukemia inhibitory factor; d factor; differentiation-stimulating factor
• UniProt Protein Name: Leukemia inhibitory factor
• Protein Family: Leukemia inhibitory factor
• UniProt Gene Name: Lif
• UniProt Synonym Gene Names: LIF; D factor
• UniProt Entry Name: LIF_MOUSE

Uniprot Description

LIF: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. Belongs to the LIF/OSM family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: growth factor activity; leukemia inhibitory factor receptor binding; cytokine activity; receptor binding

Biological Process: transcription from RNA polymerase II promoter; maternal process involved in pregnancy; negative regulation of hormone secretion; leukemia inhibitory factor signaling pathway; tyrosine phosphorylation of Stat3 protein; decidualization; positive regulation of adrenocorticotropic hormone secretion; positive regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; astrocyte differentiation; regulation of cell differentiation; negative regulation of meiosis; positive regulation of cell proliferation; positive regulation of macrophage differentiation; muscle morphogenesis; positive regulation of astrocyte differentiation; positive regulation of peptidyl-serine phosphorylation of STAT protein; organ regeneration; stem cell maintenance; stem cell differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of tyrosine phosphorylation of Stat1 protein; negative regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; retina development in camera-type eye; neuron development; positive regulation of transcription from RNA polymerase II promoter; immune response; blood vessel remodeling; alveolus development; embryo implantation; lung development