LIF recombinant protein

Mouse LIF protein, C-His tag

Cat. Num. AAA9719051

• Purity: >98% as determined by SDS-PAGE
• Synonyms: LIF; Mouse LIF protein; C-His tag; Leukemia inhibitory factor; Differentiation-stimulating factor; lif; D factor; LIF recombinant protein

• Host: E Coli
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4.
• Sequence Positions: 186aa
• Sequence: Ser23-Phe202

• Endotoxin: <0.01EU per ug of the protein as determined by the LAL method.
• Protein Construction: Amino acid sequence derived from Mouse LIF protein (P09056) (Ser23-Phe202) was expressed, was expressed with a polyhistidine tag at the C-terminus.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse LIF protein using the buffer we provided not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized Mouse LIF protein product should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig. SDS-PAGE analysis of Mouse LIF protein, C-His tag.)

Related Product Information for LIF recombinant protein

Recombinant Mouse LIF protein expressed in E Coli.
Background: Leukemia inhibitory factor (LIF) is a 20 kD protein that belongs to the IL-6 receptor family, is a pleiotropic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

Product Categories/Family for LIF recombinant protein

Cell Culture

NCBI and Uniprot Product Information

• NCBI GI #: 88014736
• NCBI GeneID: 16878
• NCBI Accession #: NP_001034626.1
• NCBI GenBank Nucleotide #: NM_001039537.2
• UniProt Accession #: P09056
• Molecular Weight: 22,287 Da
• NCBI Official Full Name: leukemia inhibitory factor isoform b
• NCBI Official Synonym Full Names: leukemia inhibitory factor
• NCBI Official Symbol: Lif
• NCBI Protein Information: leukemia inhibitory factor; d factor; differentiation-stimulating factor
• UniProt Protein Name: Leukemia inhibitory factor
• Protein Family: Leukemia inhibitory factor
• UniProt Gene Name: Lif
• UniProt Synonym Gene Names: LIF; D factor
• UniProt Entry Name: LIF_MOUSE

Uniprot Description

LIF: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. Belongs to the LIF/OSM family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: growth factor activity; leukemia inhibitory factor receptor binding; cytokine activity; receptor binding

Biological Process: transcription from RNA polymerase II promoter; maternal process involved in pregnancy; negative regulation of hormone secretion; leukemia inhibitory factor signaling pathway; tyrosine phosphorylation of Stat3 protein; decidualization; positive regulation of adrenocorticotropic hormone secretion; positive regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; astrocyte differentiation; regulation of cell differentiation; negative regulation of meiosis; positive regulation of cell proliferation; positive regulation of macrophage differentiation; muscle morphogenesis; positive regulation of astrocyte differentiation; positive regulation of peptidyl-serine phosphorylation of STAT protein; organ regeneration; stem cell maintenance; stem cell differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of tyrosine phosphorylation of Stat1 protein; negative regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; retina development in camera-type eye; neuron development; positive regulation of transcription from RNA polymerase II promoter; immune response; blood vessel remodeling; alveolus development; embryo implantation; lung development