Frizzled-5 / FZD5 Recombinant Protein | FZD5 recombinant protein

Recombinant Mouse Frizzled-5 / FZD5 Protein (Fc tag)

Cat. Num. AAA2546794

• Gene Names: Fzd5; Fz5; Fz-5; mFz5; AI427138; 5330434N09Rik
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: Frizzled-5 / FZD5; Recombinant Mouse Frizzled-5 / FZD5 Protein (Fc tag); FZD5; FZD5 recombinant protein

• Host: CHO Stable Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 585

• Application Notes: The recombinant mouse FZD5 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 382 amino acids and has a predicted molecular mass of 42.9 kDa. The apparent molecular mass of the protein is approximately 52-56 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Ala 27
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for FZD5 recombinant protein

Background: Wnt signaling is involved in a variety of embryonic development processes of nonvertebrates and vertebrates, where it determines cell motility, cell polarity, differentiation, proliferation and apoptosis, as well as formation of neural synapses. Various homologs of the Wingless protein, termed WNT factors, represent key mediators and act through a receptor complex comprised of members of the Frizzled and low density lipoprotein-related receptors (LRP). 19 WNTs, 10 Frizzled, and 2 LRP proteins have been identified. Frizzled is a family of G protein-coupled receptor proteins consisting of a divergent signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail.Frizzled 5 (FZD5) is believed to be the receptor for the Wnt5A ligand, and also interactions with Wnt10B, Wnt2B, and Wnt 7A functionally. Recent studies of WNT5A/Frizzled-5 signaling have revealed an unexpected and novel role in orchestrating adaptive immunity in response to microbial stimulation. In addition, FZD5 is also implicated in the survival of mature neurons in the parafascicular nucleus of the thalamus.

Description: A DNA sequence encoding the mouse FZD5 (Q9EQD0) (Met1-Pro167) was expressed with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 111160873
• NCBI GeneID: 14367
• NCBI Accession #: NP_001036124.1
• NCBI GenBank Nucleotide #: NM_001042659.1
• UniProt Accession #: Q9EQD0; O08975; Q8BMR2; Q8CHK9; G5E8F0
• Molecular Weight: 64,138 Da
• NCBI Official Full Name: frizzled-5
• NCBI Official Synonym Full Names: frizzled class receptor 5
• NCBI Official Symbol: Fzd5
• NCBI Official Synonym Symbols: Fz5; Fz-5; mFz5; AI427138; 5330434N09Rik
• NCBI Protein Information: frizzled-5
• UniProt Protein Name: Frizzled-5
• Protein Family: Frizzled
• UniProt Gene Name: Fzd5
• UniProt Synonym Gene Names: Fz-5; mFz5

Uniprot Description

FZD5: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta- catenin pathway. Interacts with GOPC. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: GPCR, Fz/Smo family; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 1 C2|1 32.74 cM

Cellular Component: cell surface; early endosome; Golgi apparatus; integral to membrane; perinuclear region of cytoplasm; plasma membrane; tight junction

Molecular Function: G-protein coupled receptor activity; protein binding; protein kinase binding; ubiquitin protein ligase binding; Wnt receptor activity; Wnt-protein binding

Biological Process: angiogenesis; cell maturation; determination of anterior/posterior axis, embryo; embryonic axis specification; embryonic camera-type eye development; embryonic camera-type eye morphogenesis; negative regulation of cell proliferation; positive regulation of interferon-gamma production; positive regulation of JNK activity; positive regulation of T cell cytokine production; positive regulation of transcription from RNA polymerase II promoter; post-embryonic camera-type eye development; Spemann organizer formation; T cell differentiation in the thymus; vasculature development; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin