Typical Testing Data/Standard Curve (for reference only)
Mouse IL-23 ELISA Kit | IL-23 elisa kit
IL-23 (Mouse) ELISA
Reactivity
Mouse
Synonyms
IL-23; IL-23 (Mouse) ELISA; IL-23 elisa kit
Reactivity
Mouse
Sequence Length
196
Samples
Serum, plasma, body fluids, tissue iysates or cell culture supernatants
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
<2pg/ml
Intra-assay Precision
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The %CV of intra-assay is < 10%
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. The %CV of inter-assay is < 10%
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL-23 elisa kit
Intended Uses: For detection of IL-23 in mouse serum, plasma, body fluids, tissue Iysates or cell culture supernates. For research use only,
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-23 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL-23 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-23 amount of sample captured in plate. Read the 0.0. absorbance at 450nm in a microplate reader, and then the concentration of IL-23 can be calculated
Background/Introduction: Interleukin-23 subunit alpha(IL·23) is a 189-amino acid protein that in humans IS encoded by the 1L23A gene. It is composed of the IL 12 p40 subunit and the p19 subunit (IL23A), and is produced by dendritic cells and macrophages. Moreover, IL·23 is stimulated by Danger Signals, including cell debris, and directs memory T cells to Th17 response. It is an important part of the inflammatory response against infection. Recently, IL-23 has been implicated in the development of cancerous tumors. Langowski et al. (2006) showed that 1L23 is an important molecular link between tumor-promoter proinflammatory processes and the failure of the adaptive immune surveillance to infiltrate tumors.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-23 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL-23 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-23 amount of sample captured in plate. Read the 0.0. absorbance at 450nm in a microplate reader, and then the concentration of IL-23 can be calculated
Background/Introduction: Interleukin-23 subunit alpha(IL·23) is a 189-amino acid protein that in humans IS encoded by the 1L23A gene. It is composed of the IL 12 p40 subunit and the p19 subunit (IL23A), and is produced by dendritic cells and macrophages. Moreover, IL·23 is stimulated by Danger Signals, including cell debris, and directs memory T cells to Th17 response. It is an important part of the inflammatory response against infection. Recently, IL-23 has been implicated in the development of cancerous tumors. Langowski et al. (2006) showed that 1L23 is an important molecular link between tumor-promoter proinflammatory processes and the failure of the adaptive immune surveillance to infiltrate tumors.
Product Categories/Family for IL-23 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,986 Da
NCBI Official Full Name
interleukin 23
NCBI Official Synonym Full Names
Interleukin 23, alpha subunit p19
NCBI Official Symbol
Il23a
NCBI Protein Information
interleukin-23 subunit alpha; IL-23 subunit alpha; IL-23-A; IL-23p19; interleukin-23 subunit p19
UniProt Protein Name
Interleukin-23 subunit alpha
UniProt Gene Name
Il23a
UniProt Synonym Gene Names
IL-23 subunit alpha; IL-23-A; IL-23p19
UniProt Entry Name
IL23A_RAT
NCBI Description
mouse homolog is a pro-inflammatory cytokine; high levels of expression may be associated with rheumatoid arthritis, psoriasis, and multiple sclerosis [RGD, Feb 2006]
Uniprot Description
Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis ().
Research Articles on IL-23
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Product Notes
The Mouse IL-23 il23a (Catalog #AAA494788) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494788 ELISA Kit recognizes Mouse IL-23. It is sometimes possible for the material contained within the vial of "IL-23, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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