Typical Testing Data/Standard Curve (for reference only)
ICAM-1 / CD54 elisa kit
ICAM-1 / CD54 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit is based on a sandwich enzyme-linked immune-sorbent assay technology. An analyte-specific polyclonal antibody is pre-coated onto 96-wett plates. The biotin conjugated second antibody is used as detection antibody. During the first incubation the standards and samples react with the analyte-specific pre-coated antibody. In a second incubation the biotin conjugated detection antibody completes the sandwich. After washing wi th wash buffer the TMB substrate is added to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after add ing acidic stop solution. The optical density of the yellow color is proportional to the analyte captured in plate. Read the 0.0,. absorbance at 450nm in a microtiterplale reader, and catcutate the concentration of the analyte in the sample by taking into consideration the dilution factor of the sample!!Background/Introduction: Interleukin-17(IL-17 or IL-17A) originally identified as a transcript from a rodent T-cell hybridoma by Rouvier et ai, in 1993. The IL-7 gene maps to human chromosome 2q31. It is the founding member of a group of cytokines called the IL-17 family, and secreted primarily by activated T cells. IL-17 shows high homology to virallL-17 encoded by an open reading frame of the T-Iymphotropic rhadinovirus Herpesvirus saimiri. All members of the IL-17 family have a similar protein structure, with four highly conserved cysteine residues critical to their 3-dimensional shape, yet they have no sequence similarity to any other known cytokines. The most notable role of IL-17 is it involvement in inducing and mediating proinflammatory responses. IL-17F has been well characterized both in vitro and in vivo, and has been shown to have a pro-inflammatory role in asthma.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
Uniprot Description
ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta.
Protein type: Cell adhesion; Immunoglobulin superfamily; Membrane protein, integral
Chromosomal Location of Human Ortholog: 19p13.3-p13.2
Cellular Component: cell surface; external side of plasma membrane; extracellular space; focal adhesion; immunological synapse; integral to plasma membrane; lipid raft; membrane; plasma membrane
Molecular Function: integrin binding; protein binding; receptor activity; transmembrane receptor activity; viral receptor activity
Biological Process: activation of NF-kappaB transcription factor; acute inflammatory response to antigenic stimulus; adhesion to host; cell adhesion; cell adhesion mediated by integrin; cell aging; cellular response to nutrient levels; cytokine and chemokine mediated signaling pathway; entry of virus into host cell; extracellular matrix organization and biogenesis; heterophilic cell adhesion; leukocyte adhesion; leukocyte migration; membrane to membrane docking; negative regulation of calcium ion transport; ovarian follicle development; positive regulation of actin filament polymerization; positive regulation of cellular extravasation; positive regulation of GTPase activity; positive regulation of nitric oxide biosynthetic process; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of vasoconstriction; regulation of cell adhesion; regulation of cell shape; regulation of immune response; regulation of leukocyte mediated cytotoxicity; response to amino acid stimulus; response to amphetamine; response to copper ion; response to drug; response to ethanol; response to ionizing radiation; response to organic cyclic substance; response to sulfur dioxide; sensory perception of sound; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; T cell antigen processing and presentation; virion attachment, binding of host cell surface receptor
Disease: Malaria, Susceptibility To
Research Articles on ICAM-1 / CD54
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Product Notes
The ICAM-1 / CD54 icam1 (Catalog #AAA495229) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ICAM-1 / CD54, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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