Typical Testing Data/Standard Curve (for reference only)
Human MMP-7 ELISA Kit | MMP-7 elisa kit
MMP-7 ELISA
Reactivity
Human
Synonyms
MMP-7; MMP-7 ELISA; MMP-7 elisa kit
Reactivity
Human
Samples
Rat Serum, Plasma, Body Fluids, Tissue Iysates Or Cell Culture Supernates
Assay Type
Sandwich
Detection Range
625 pglml. 40,000 pglml
Sensitivity
<10pglml
Intra-assay Precision
Three samples of known concentration were tested twenty tim~s on one plate to assess intra-assay precision. The %CV of intra-assay is < 10%
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. The %CV of inter-assay is < 10%
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MMP-7 elisa kit
Intended Uses: For detection of MMP-2 in Rat serum, plasma, body fluids, tissue Iysates or cell culture superna\es. For research use only
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MMP-2 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MMP-2 polyclonal antibody was used as detection antibodies. The standards. test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MMP-2 amount of sample captured in plate. Read the 0.0. absorbance at 450nm in a microplate reader. and then the concentration of MMP-2 can be calculated!!Background/Introduction: Type IV collagenase, 72-kO, is officially designated matrix metalioproteinase-2 (MMP2). It is also known as gela\inase. 72-kD. It is a metailoproteinase that specifIcally cleaves type IV collagen, the major structural component of basement membranes_ MMP-2 is a likely effector of endometrial menstrual breakdown. It plays a rote in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response, Kenny et al. (2008) found that expression of MMP2 was induced upon attachment of ovarian cancer (OvCa) cells to mesothelium.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MMP-2 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MMP-2 polyclonal antibody was used as detection antibodies. The standards. test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MMP-2 amount of sample captured in plate. Read the 0.0. absorbance at 450nm in a microplate reader. and then the concentration of MMP-2 can be calculated!!Background/Introduction: Type IV collagenase, 72-kO, is officially designated matrix metalioproteinase-2 (MMP2). It is also known as gela\inase. 72-kD. It is a metailoproteinase that specifIcally cleaves type IV collagen, the major structural component of basement membranes_ MMP-2 is a likely effector of endometrial menstrual breakdown. It plays a rote in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response, Kenny et al. (2008) found that expression of MMP2 was induced upon attachment of ovarian cancer (OvCa) cells to mesothelium.
Product Categories/Family for MMP-7 elisa kit
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Product Notes
The Human MMP-7 (Catalog #AAA494798) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494798 ELISA Kit recognizes Human MMP-7. It is sometimes possible for the material contained within the vial of "MMP-7, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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