Mouse IL-23 ELISA Kit | IL23A elisa kit

Mouse IL-23 PicoKine ELISA Kit

Cat. Num. AAA175887

• Gene Names: Il23a; p19; IL-23
• Reactivity: Mouse
• Synonyms: IL-23; Mouse IL-23 PicoKine ELISA Kit; Interleukin-23 subunit alpha; interleukin 23; alpha subunit p19; IL 23; IL 23A; IL-23 subunit alpha; IL-23-A; IL-23p19; Il23a; IL23A_HUMAN; IL23P19; Interleukin 23 alpha subunit p19; Interleukin-23 subunit p19; JKA3 induced upon T cell activation; P19; SGRF; IL23A elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse IL-23
• Sequence Length: 196

• Samples: Cell culture supernates, serum and plasma (heparin)
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 2pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse IL-23 PicoKine ELISA Kit standard curve )

Related Product Information for IL23A elisa kit

Principle of the assay: mouse IL-23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-23 has been precoated onto 96-well plates. Standards(sf21, (M23-S335)+(L20-A196)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-23 amount of sample captured in plate.
Background: Interleukin-23 subunit alpha(IL-23) is a protein that in humans is encoded by the IL23A gene. IL-23 is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19 (the IL-23 alpha subunit). IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. The International Radiation Hybrid Mapping Consortium mapped the p19 gene to chromosome 12 (stSG47812).

NCBI and Uniprot Product Information

• NCBI GI #: 81881842
• NCBI GeneID: 83430
• UniProt Accession #: Q9EQ14
• Molecular Weight: 22,071 Da
• NCBI Official Full Name: Interleukin-23 subunit alpha
• NCBI Official Synonym Full Names: interleukin 23, alpha subunit p19
• NCBI Official Symbol: Il23a
• NCBI Official Synonym Symbols: p19; IL-23
• NCBI Protein Information: interleukin-23 subunit alpha; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin-23 subunit p19
• UniProt Protein Name: Interleukin-23 subunit alpha
• Protein Family: Interleukin
• UniProt Gene Name: Il23a
• UniProt Synonym Gene Names: IL-23 subunit alpha; IL-23-A; IL-23p19
• UniProt Entry Name: IL23A_MOUSE

Uniprot Description

IL23A: Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak- Stat signaling cascade, stimulates memory rather than naive T- cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. Belongs to the IL-6 superfamily.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; cytokine activity; interleukin-23 receptor binding

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; negative regulation of interleukin-10 production; positive regulation of T-helper 1 type immune response; positive regulation of osteoclast differentiation; positive regulation of T cell mediated cytotoxicity; positive regulation of interleukin-12 production; tissue remodeling; positive regulation of NK T cell proliferation; positive regulation of tyrosine phosphorylation of Stat4 protein; positive regulation of activated T cell proliferation; defense response to Gram-negative bacterium; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; T cell proliferation; positive regulation of T cell proliferation; inflammatory response; defense response to virus; positive regulation of interleukin-17 production; positive regulation of memory T cell differentiation; positive regulation of NK T cell activation; positive regulation of natural killer cell proliferation; immune system process; positive regulation of tumor necrosis factor production; regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of interferon-gamma production; positive regulation of tyrosine phosphorylation of Stat5 protein; innate immune response; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of defense response to virus by host