Typical Testing Data/Standard Curve (for reference only)
Mouse Fibronectin ELISA Kit | fn1 elisa kit
Fibronectin (Mouse) ELISA
Gene Names
fn1; fibronectin
Reactivity
Mouse
Synonyms
Fibronectin; Fibronectin (Mouse) ELISA; fn1 elisa kit
Reactivity
Mouse
Sequence Length
2481
Samples
Serum, plasma, body fluids, tissue iysates or cell culture supernatants
Assay Type
Sandwich
Detection Range
156pg/ml-10,OOOpg/ml
Sensitivity
<15pg/ml
Intra-assay Precision
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The %CV of intra-assay is < 10%
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. The %CV of inter-assay is < 10%
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for fn1 elisa kit
Intended Uses: For detection of Fibronectin in mouse serum, plasma, body fl uids, tissue Iysates or cell culture supernatants. For resea rch use on ly,
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Fibronectin polyclonal antibody is pre-coated onto 96-well plates. And the biotin conjugated anti- Fibronectin polyclonal antibody is used as detection antibodies. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB is cata lyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Fibronectin amount of sample captured in plate. Read the 0.0. absorbance at 4S0nm in a microplate reader, and then the concentration of Fibronectin can be ca lculated.
Background/Introduction: Fibronectin is a high-molecular weight (-440kDa) glycoprotein of the extracellular metri x. belongs to a fam ily of high molecular weight glycoproteins that are present on cell surfaces, in extracellular flu ids. connective tissues, and basement membranes. Two major forms of fibronectin exist: a plasma soluble form and a, cellul ar form. It plays a major role in cell adhesion, growth. migration, and differentiation, and it is important for processes such as wound healing and embryonic development. Fibronectin has been implicated in carcinoma development, and also acts as a potential biomarker for radioresistance.
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Fibronectin polyclonal antibody is pre-coated onto 96-well plates. And the biotin conjugated anti- Fibronectin polyclonal antibody is used as detection antibodies. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB is cata lyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Fibronectin amount of sample captured in plate. Read the 0.0. absorbance at 4S0nm in a microplate reader, and then the concentration of Fibronectin can be ca lculated.
Background/Introduction: Fibronectin is a high-molecular weight (-440kDa) glycoprotein of the extracellular metri x. belongs to a fam ily of high molecular weight glycoproteins that are present on cell surfaces, in extracellular flu ids. connective tissues, and basement membranes. Two major forms of fibronectin exist: a plasma soluble form and a, cellul ar form. It plays a major role in cell adhesion, growth. migration, and differentiation, and it is important for processes such as wound healing and embryonic development. Fibronectin has been implicated in carcinoma development, and also acts as a potential biomarker for radioresistance.
Product Categories/Family for fn1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
272,680 Da
NCBI Official Full Name
fibronectin
NCBI Official Synonym Full Names
fibronectin 1<
NCBI Official Symbol
fn1
NCBI Official Synonym Symbols
fibronectin
NCBI Protein Information
fibronectin; FN; gene C
UniProt Protein Name
Fibronectin
Protein Family
UniProt Gene Name
fn1
UniProt Synonym Gene Names
FN
UniProt Entry Name
FINC_XENLA
Uniprot Description
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. May be involved in osteoblast compaction ().
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Product Notes
The Mouse fn1 fn1 (Catalog #AAA494774) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494774 ELISA Kit recognizes Mouse fn1. It is sometimes possible for the material contained within the vial of "Fibronectin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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