Typical Testing Data/Standard Curve (for reference only)
Human TNFsR II ELISA Kit | TNFsR II elisa kit
TNFsR II ELISA
Reactivity
Human
Synonyms
TNFsR II; TNFsR II ELISA; TNFsR II elisa kit
Reactivity
Human
Samples
Human Serum, Plasma, Body Fluids, Tissue Lysates Or Cell Culture Supernates
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
<2pg/ml
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TNFsR II elisa kit
Intended Uses: For detection of TIMP-3 in human serum, plasma, body fluids, tissue lysates or cell culture supernates. For research use only.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TIMP-3 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TIMP-3 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TIMP-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TIMP-3 can be calculated!!Background/Introduction: Metalloproteinase inhibitor 3 is an protein that in humans is encoded by the TIMP3 gene, which maps to to chromosome 22. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. The balance of MMP and TIMP determines the net migratory capacity of DCs. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TIMP-3 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TIMP-3 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TIMP-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TIMP-3 can be calculated!!Background/Introduction: Metalloproteinase inhibitor 3 is an protein that in humans is encoded by the TIMP3 gene, which maps to to chromosome 22. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. The balance of MMP and TIMP determines the net migratory capacity of DCs. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy.
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Product Notes
The Human TNFsR II (Catalog #AAA494826) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494826 ELISA Kit recognizes Human TNFsR II. It is sometimes possible for the material contained within the vial of "TNFsR II, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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