Typical Testing Data/Standard Curve (for reference only)
Mouse IL-7 ELISA Kit | IL-7 elisa kit
IL-7 (Mouse) ELISA
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-23 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-23 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-23 amount of sample captured in plate. Read the 0.0. absorbance at 4s0nm in a microplate reader, and then the concentration of IL-23 can be calculated!!Background/Introduction: Interleukin-23 subunit alpha(IL-23) is a 1S9-amino acid protein that in humans is encoded by the IL23A gene. It is composed of the IL 12 p40 subunit and the p19 subunit (IL23A). and is produced by dendritic cells and macrophages. Moreover, IL-23 is stimulated by Danger Signals, including cell debris, and directs memory T cells to Th17 response. It is an important part of the inflammatory response against infection. Recently, IL-23 has been implicated in the development of cancerous tumors. Langowski et al. (2006) showed that IL23 is an important molecular link between tumor-promoter proinflammatory processes and the failure of the adaptive immune surveillance to infiltrate tumors.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.[provided by RefSeq, Dec 2010]
Uniprot Description
IL7: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Belongs to the IL-7/IL-9 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis; Secreted; Cell development/differentiation; Secreted, signal peptide; Cell cycle regulation; Cytokine
Chromosomal Location of Human Ortholog: 8q12-q13
Cellular Component: extracellular space; extracellular region
Molecular Function: interleukin-7 receptor binding; protein binding; growth factor activity; cytokine activity
Biological Process: organ morphogenesis; T cell lineage commitment; cell-cell signaling; positive regulation of T cell differentiation; regulation of gene expression; homeostasis of number of cells within a tissue; positive regulation of cell proliferation; negative regulation of catalytic activity; positive regulation of B cell proliferation; positive regulation of organ growth; humoral immune response; negative regulation of apoptosis; bone resorption
Research Articles on IL-7
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Product Notes
The Mouse IL-7 il7 (Catalog #AAA494786) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494786 ELISA Kit recognizes Mouse IL-7. It is sometimes possible for the material contained within the vial of "IL-7, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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