Mouse CD6 ELISA Kit | CD6 elisa kit

Mouse CD6 PicoKine ELISA Kit

Cat. Num. AAA177177

• Reactivity: Mouse
• Synonyms: CD6; Mouse CD6 PicoKine ELISA Kit; T-cell differentiation antigen CD6; CD6 molecule; CD_antigen; CD6 antigen Tp120; CD6_HUMAN; FLJ44171; OX52; T cell differentiation antigen CD6; T12; TP120; CD6 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse CD6
• Sequence Length: 665

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse CD6 PicoKine ELISA Kit standard curve )

Related Product Information for CD6 elisa kit

Principle of the assay: mouse CD6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD6 has been precoated onto 96-well plates. Standards(NSO, L18-G396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD6 amount of sample captured in plate.
Background: CD6(Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What's more, this gene may be associated with susceptibility to multiple sclerosis.

NCBI and Uniprot Product Information

• NCBI GI #: 341940337
• NCBI GeneID: 12511
• UniProt Accession #: Q61003; Q60679; Q61004; Q8BGK1
• Molecular Weight: 67,892 Da
• NCBI Official Full Name: T-cell differentiation antigen CD6
• NCBI Official Synonym Full Names: CD6 antigen
• NCBI Official Symbol: Cd6
• NCBI Protein Information: T-cell differentiation antigen CD6
• UniProt Protein Name: T-cell differentiation antigen CD6
• Protein Family: CD63 antigen
• UniProt Gene Name: Cd6
• UniProt Entry Name: CD6_MOUSE

Uniprot Description

CD6: a type I membrane protein involved in cell adhesion. Binds to CD166. Expressed on thymocytes, peripheral T cells, a subset of B cells, and a subset of neurons. Mediates the binding of developing thymocytes with thymic epithelial cells. CD6- T cells are less autoreactive than CD6+ T cells. Phosphorylated on tyrosines and serines following T cell antigen receptor signaling. Five splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Cell surface

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; glycoprotein binding; protein kinase binding; scavenger receptor activity

Biological Process: positive regulation of protein amino acid phosphorylation; cell adhesion