Human IL-31 ELISA Kit | IL31 elisa kit

Human IL-31 PicoKine ELISA Kit

Cat. Num. AAA177368

• Gene Names: IL31; IL-31
• Reactivity: Human
• Synonyms: IL-31; Human IL-31 PicoKine ELISA Kit; Interleukin-31; interleukin 31; IL31; IL31_HUMAN; IL31 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human IL-31
• Sequence Length: 164

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human IL-31 PicoKine ELISA Kit standard curve )

Related Product Information for IL31 elisa kit

Principle of the assay: human IL-31 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-31 has been precoated onto 96-well plates. Standards(E.coli, S24-T164) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-31 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-31 amount of sample captured in plate.
Background: Interleukin-31, also known as IL-31 is a protein that in humans is encoded by the IL31 gene. This gene is mapped to 12q24.31. IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA and OSMR that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma. IL31 may function in skin immunity.

NCBI and Uniprot Product Information

• NCBI GI #: 74709232
• NCBI GeneID: 386653
• UniProt Accession #: Q6EBC2; A2RUQ1
• Molecular Weight: 18,205 Da
• NCBI Official Full Name: Interleukin-31
• NCBI Official Synonym Full Names: interleukin 31
• NCBI Official Symbol: IL31
• NCBI Official Synonym Symbols: IL-31
• NCBI Protein Information: interleukin-31
• UniProt Protein Name: Interleukin-31
• Protein Family: Interleukin
• UniProt Gene Name: IL31
• UniProt Synonym Gene Names: IL-31
• UniProt Entry Name: IL31_HUMAN

NCBI Description

IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma (Dillon et al., 2004 [PubMed 15184896]).[supplied by OMIM, Mar 2008]

Uniprot Description

IL31: Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: extracellular space

Molecular Function: cytokine activity

Biological Process: immune system process