Human FLRG/FSTL3 ELISA Kit | FSTL3 elisa kit

Human FLRG/FSTL3 PicoKine ELISA Kit

Cat. Num. AAA177118

• Gene Names: FSTL3; FLRG; FSRP
• Reactivity: Human
• Synonyms: FLRG/FSTL3; Human FLRG/FSTL3 PicoKine ELISA Kit; Follistatin-related protein 3; follistatin-like 3 (secreted glycoprotein); FLRG; follistatin-like 3; follistatin-like 3 glycoprotein; Follistatin-like protein 3; Follistatin-related gene protein; FSRP; Fstl3; FSTL3_HUMAN; FSTL3 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human FLRG
• Sequence Length: 263

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA), urine and human milk
• Assay Type: Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human FLRG/FSTL3 PicoKine ELISA Kit standard curve )

Related Product Information for FSTL3 elisa kit

Principle of the assay: human FLRG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FLRG has been precoated onto 96-well plates. Standards(NSO, M27-V263) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FLRG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FLRG amount of sample captured in plate.
Background: FSTL3(Follistatin-Like 3) also known as FLRG or FOLLISTATIN-RELATED GENE, is a member of the follistatin-module protein family, which is composed of extracellular matrix-associated glycoproteins thought to act in a paracrine manner to bind morphogens or growth/differentiation factors and regulate their activity during development. The FSTL3 gene extends over 7 kbp and contains 5 exons. The FSTL3 gene was localized to chromosome 19p13. This gene involved in hematopoiesis and in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. It may have a role in leukemogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 23821565
• NCBI GeneID: 10272
• UniProt Accession #: O95633; A8K7E3
• Molecular Weight: 24,960 Da
• NCBI Official Full Name: Follistatin-related protein 3
• NCBI Official Synonym Full Names: follistatin-like 3 (secreted glycoprotein)
• NCBI Official Symbol: FSTL3
• NCBI Official Synonym Symbols: FLRG; FSRP
• NCBI Protein Information: follistatin-related protein 3; follistatin-like protein 3; follistatin-related gene protein
• UniProt Protein Name: Follistatin-related protein 3
• Protein Family: Follistatin-related protein
• UniProt Gene Name: FSTL3
• UniProt Synonym Gene Names: FLRG
• UniProt Entry Name: FSTL3_HUMAN

NCBI Description

Follistatin-like 3 is a secreted glycoprotein of the follistatin-module-protein family. It may have a role in leukemogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

FSTL3: Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10. A chromosomal aberration involving FSTL3 is found in a case of B-cell chronic lymphocytic leukemia. Translocation t(11;19)(q13;p13) with CCDN1. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Oncoprotein; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: nucleoplasm; Golgi apparatus; extracellular space; extracellular region; nucleus; secretory granule

Molecular Function: protein binding; fibronectin binding; activin binding

Biological Process: negative regulation of osteoclast differentiation; ossification; transcription, DNA-dependent; adrenal gland development; male gonad development; negative regulation of BMP signaling pathway; regulation of transcription from RNA polymerase II promoter; negative regulation of activin receptor signaling pathway; positive regulation of cell-cell adhesion; hemopoietic progenitor cell differentiation; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; kidney development; lung development