Human Cathepsin S ELISA Kit | CTSS elisa kit

Human Cathepsin S PicoKine ELISA Kit

Cat. Num. AAA177353

• Reactivity: Human
• Synonyms: Cathepsin S; Human Cathepsin S PicoKine ELISA Kit; cathepsin S; CTSS; CTSS elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Cathepsin S
• Sequence Length: 331

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human Cathepsin S PicoKine ELISA Kit standard curve )

Related Product Information for CTSS elisa kit

Principle of the assay: human Cathepsin S ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin S has been precoated onto 96-well plates. Standards(NSO, Q17-I331 & S109-I331) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin S is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cathepsin S amount of sample captured in plate.
Background: Cathepsin S, also known as CTSS, is a protein that in humans is encoded by the CTSS gene. A member of the peptidase C1 family, CTSS gene is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. By fluorescence in situ hybridization (FISH), this gene is mapped to 1q21.3. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.

NCBI and Uniprot Product Information

• NCBI GI #: 88984046
• NCBI GeneID: 1520
• UniProt Accession #: P25774; Q5T5I0; Q6FHS5; Q9BUG3; B4DWC9; D3DV05
• Molecular Weight: 31,626 Da
• NCBI Official Full Name: Cathepsin S
• NCBI Official Synonym Full Names: cathepsin S
• NCBI Official Symbol: CTSS
• NCBI Protein Information: cathepsin S
• UniProt Protein Name: Cathepsin S
• Protein Family: Cathepsin
• UniProt Gene Name: CTSS
• UniProt Entry Name: CATS_HUMAN

NCBI Description

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

CTSS: Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond- specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N. Belongs to the peptidase C1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.22.27

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: lysosomal lumen; extracellular space; intracellular membrane-bound organelle; membrane; lysosome; extracellular region

Molecular Function: collagen binding; proteoglycan binding; fibronectin binding; cysteine-type endopeptidase activity; laminin binding

Biological Process: extracellular matrix organization and biogenesis; adaptive immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis; collagen catabolic process; antigen processing and presentation; extracellular matrix disassembly; antigen processing and presentation of peptide antigen via MHC class I; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; immune response; positive regulation of inflammatory response