Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad3 antibody (Ser425))
Rabbit SMAD3 Polyclonal Antibody | anti-SMAD3 antibody
SMAD3 Antibody (Ser425)
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Chromatography
Synonyms
SMAD3; Polyclonal Antibody; SMAD3 Antibody (Ser425); Rabbit Polyclonal SMAD3 Antibody (Ser425); Polyclonal Smad3 antibody; Anti-Smad3 antibody; DKFZp586N0721 antibody; DKFZp686J10186 antibody; hMAD-3 antibody; hSMAD3 antibody; HSPC193 antibody; HsT17436 antibody; JV15-2 antibody; MAD homolog 3 antibody; mad homolog JV15-2 antibody; mad protein homolog antibody; MAD mothers against decapentaplegic homolog 3 antibody; Mad3 antibody; MADH3 antibody; MGC60396 antibody; Mothers against decapentaplegic homolog 3 antibody; Mothers against DPP homolog 3 antibody; SMA- and MAD-related protein 3 antibody; SMAD 3 antibody; SMAD family member 3 antibody; SMAD mothers against DPP homolog 3 antibody; anti-SMAD3 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Smad3 antibody (Ser425) detects endogenous levels of Smad3 only when phosphorylated at serine 425
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
425
Applicable Applications for anti-SMAD3 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
WB: 1:500-1:1000
WB: 1:500-1:1000
Immunogen
Smad3 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of serine 425 (C-S-S-V-SP) as the immunogen.
Cross-Reactivity
Mouse, Rat
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad3 antibody (Ser425))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad3 antibody (Ser425))
Related Product Information for anti-SMAD3 antibody
Smad3 is a receptor-regulated Smad (R-smad) which binds directly to consensus DNA-binding elements in the promoters of target genes.
Product Categories/Family for anti-SMAD3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Smad3
NCBI Official Synonym Full Names
SMAD family member 3
NCBI Official Symbol
SMAD3
NCBI Official Synonym Symbols
LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
NCBI Protein Information
mothers against decapentaplegic homolog 3
Protein Family
NCBI Description
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
Research Articles on SMAD3
Similar Products
Product Notes
The SMAD3 (Catalog #AAA5313725) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SMAD3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:50-1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the SMAD3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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