Typical Testing Data/Standard Curve (for reference only)
(Human soluble CD5 PicoKine ELISA Kit standard curve )
Human soluble CD5 ELISA Kit | CD5 elisa kit
Human soluble CD5 PicoKine ELISA Kit
Gene Names
CD5; T1; LEU1
Reactivity
Human
Synonyms
soluble CD5; Human soluble CD5 PicoKine ELISA Kit; T-cell surface glycoprotein CD5; CD5 molecule; CD 5; CD5; CD5 antigen (p56 62); CD5 antigen; CD5_HUMAN; LEU 1; LEU1; Ly12; LyA; Lymphocyte Antigen CD5; Lymphocyte antigen T1/Leu 1; Lymphocyte antigen T1/Leu-1; Lymphocyte glycoprotein T1/Leu1; OTTHUMP00000236973; p56 62; T cell surface glycoprotein CD5; T1; CD5 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human CD5
Sequence Length
495
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
93.7pg/ml-6000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Human soluble CD5 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human soluble CD5 PicoKine ELISA Kit standard curve )
Related Product Information for CD5 elisa kit
Principle of the assay: human CD5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD5 has been precoated onto 96-well plates. Standards(NSO, R25-N371) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD5 amount of sample captured in plate.
Background: CD5, also known as T1 or LEU1, is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. It is mapped to 11q12.2. Although CD5 can be found on both T cells and B cells, T cells express higher levels of CD5 than B cells. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. CD5 is upregulated on T cells upon strong activation. In the thymus, there is a correlation with CD5 expression and strength of the interaction of the T cell towards self-peptides. Nevertheless, CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins.
Background: CD5, also known as T1 or LEU1, is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. It is mapped to 11q12.2. Although CD5 can be found on both T cells and B cells, T cells express higher levels of CD5 than B cells. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. CD5 is upregulated on T cells upon strong activation. In the thymus, there is a correlation with CD5 expression and strength of the interaction of the T cell towards self-peptides. Nevertheless, CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
54,578 Da
NCBI Official Full Name
T-cell surface glycoprotein CD5
NCBI Official Synonym Full Names
CD5 molecule
NCBI Official Symbol
CD5
NCBI Official Synonym Symbols
T1; LEU1
NCBI Protein Information
T-cell surface glycoprotein CD5; CD5 antigen (p56-62); lymphocyte antigen T1/Leu-1
UniProt Protein Name
T-cell surface glycoprotein CD5
Protein Family
UniProt Gene Name
CD5
UniProt Synonym Gene Names
LEU1
UniProt Entry Name
CD5_HUMAN
Uniprot Description
CD5: a surface glycoprotein expressed on T cells and B-1 B cells. Rapidly recruited to the immunological synapse, lowering the response of the T cell antigen receptor. CD5 interacts with CD72/LYB-2. Promotes B-cell survival through stimulation of autocrine IL-10 production. Contains 3 SRCR domains.
Protein type: Membrane protein, integral; Cell surface
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane
Molecular Function: protein binding; transmembrane receptor activity; receptor activity; scavenger receptor activity
Biological Process: cell proliferation; receptor-mediated endocytosis; T cell costimulation; cell recognition
Research Articles on CD5
Similar Products
Product Notes
The Human CD5 cd5 (Catalog #AAA177219) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177219 ELISA Kit recognizes Human CD5. It is sometimes possible for the material contained within the vial of "soluble CD5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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