Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFRSF18/GITR PicoKine ELISA Kit standard curve )
Mouse TNFRSF18/GITR ELISA Kit | TNFRSF18 elisa kit
Mouse TNFRSF18/GITR PicoKine ELISA Kit
Gene Names
Tnfrsf18; AITR; Gitr
Reactivity
Mouse
Synonyms
TNFRSF18/GITR; Mouse TNFRSF18/GITR PicoKine ELISA Kit; Tumor necrosis factor receptor superfamily member 18; tumor necrosis factor receptor superfamily; member 18; Activation inducible TNFR family receptor; AITR; GITR; GITR D; Glucocorticoid induced TNFR related protein; TNFRSF18 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse TNFRSF18
Sequence Length
228
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFRSF18/GITR PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFRSF18/GITR PicoKine ELISA Kit standard curve )
Related Product Information for TNFRSF18 elisa kit
Principle of the assay: mouse TNFRSF18 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF18 has been precoated onto 96-well plates. Standards(NSO, S22-H153) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF18 amount of sample captured in plate.
Background: Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. This gene is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Background: Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. This gene is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
14,106 Da
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 18
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 18
NCBI Official Symbol
Tnfrsf18
NCBI Official Synonym Symbols
AITR; Gitr
NCBI Protein Information
tumor necrosis factor receptor superfamily member 18; glucocorticoid-induced TNFR-related protein
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 18
Protein Family
UniProt Gene Name
Tnfrsf18
UniProt Synonym Gene Names
Gitr
UniProt Entry Name
TNR18_MOUSE
Uniprot Description
TNFRSF18: Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, cytokine; Apoptosis
Cellular Component: membrane; integral to plasma membrane; extracellular region; plasma membrane; integral to membrane; external side of plasma membrane
Molecular Function: protein binding
Biological Process: apoptosis
Research Articles on TNFRSF18
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Product Notes
The Mouse TNFRSF18 tnfrsf18 (Catalog #AAA177257) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177257 ELISA Kit recognizes Mouse TNFRSF18. It is sometimes possible for the material contained within the vial of "TNFRSF18/GITR, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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