Mouse IL-3 CLIA Kit | IL-3 clia kit

IL-3 (Murine) LumiAb ELISA Kit

Cat. Num. AAA9510840

• Gene Names: Il3; BPA; PSF; HCGF; Il-3; MCGF; Csfmu
• Reactivity: Mouse
• Synonyms: IL-3; IL-3 (Murine) LumiAb ELISA Kit; Murine IL-3; Interleukin-3; Hematopoietic growth factor; Mast cell growth factor; Multipotential colony-stimulating factor; P-cell-stimulating factor; MCGF; Csfmu; IL-3 clia kit

• Reactivity: Mouse
• Specificity: The LumiAb Murine IL-21 ELISA is capable of recognizing both recombinant and naturally produced Murine IL-21 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 166

• Assay Type: Quantitative Sandwich
• Sensitivity: 11 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-3 clia kit

Principle of the Assay: The LumiAb Murine IL-21 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Murine IL-21 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Murine IL-21 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Murine IL-21 or Interleukin-21 is a 146 amino acid cytokine protein expressed from chromosome 3. After initial synthesis and translocation, the 17 residue signal sequence is cleaved, allowing the 129 residue IL-21 peptide to properly fold and mature. IL-21, a cytokine with immunoregulatory activity, may serve to promote the transition between innate and adaptive immunity, induce the production of IgG1 and IgG3 in B-cells, and possibly play a role in the proliferation and maturation of natural killer (NK) cells in synergy with IL-15. Also, IL-21 may regulate proliferation of mature B-and T-cells in response to activating stimuli. IL-21, interacting with its family members, IL-15 and IL-18, can stimulate IFN-gamma production in T-cells and NK cells. During T-cell mediated immune responses, IL-21 may inhibit dendritic cells (DC) activation and maturation.

Product Categories/Family for IL-3 clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 31982791
• NCBI GeneID: 16187
• NCBI Accession #: NP_034686.2
• NCBI GenBank Nucleotide #: NM_010556.4
• UniProt Accession #: P01586
• NCBI Official Full Name: interleukin-3
• NCBI Official Synonym Full Names: interleukin 3
• NCBI Official Symbol: Il3
• NCBI Official Synonym Symbols: BPA; PSF; HCGF; Il-3; MCGF; Csfmu
• NCBI Protein Information: interleukin-3
• UniProt Protein Name: Interleukin-3
• UniProt Gene Name: Il3
• UniProt Synonym Gene Names: Csfmu; Il-3; IL-3; MCGF
• UniProt Entry Name: IL3_MOUSE

Uniprot Description

IL3: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Belongs to the IL-3 family.

Protein type: Apoptosis; Cell cycle regulation; Cytokine; Oncoprotein; Secreted; Secreted, signal peptide

Cellular Component: extracellular space

Molecular Function: cytokine activity; growth factor activity; interleukin-3 receptor binding

Biological Process: activation of JAK protein; cytokine and chemokine mediated signaling pathway; hemopoiesis; monocyte differentiation; myeloid cell apoptosis; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of mast cell apoptosis; positive regulation of cell proliferation; positive regulation of JNK cascade; positive regulation of myeloid leukocyte differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein amino acid phosphorylation; regulation of cell growth; regulation of gene expression; regulation of glycolysis; regulation of protein amino acid dephosphorylation; response to hormone stimulus; response to hypoxia