PD-L2 ELISA Pair Set | PDCD1LG2 elisa pair set

PD-L2 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118227

• Synonyms: PD-L2; PD-L2 Matched ELISA Antibody Pair Set; Human; Human PD-L2/B7-DC/CD273 ELISA Pair Set; programmed cell death 1 ligand 2; B7-DC Matched ELISA Antibody Pair Set; B7DC Matched ELISA Antibody Pair Set; bA574F11.2 Matched ELISA Antibody Pair Set; Btdc Matched ELISA Antibody Pair Set; CD273 Matched ELISA Antibody Pair Set; PDCD1L2 Matched ELISA Antibody Pair Set; PDL2 Matched ELISA Antibody Pair Set; PDCD1LG2 elisa pair set

• Specificity: Quantitative determination of Human PD-L2

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 25-1600pg/mL
• Sensitivity: 25pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of rabbit anti-Human PD-L2/B7-DC/CD273 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human PD-L2/B7-DC/CD273 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 70 ng of recombinant Human PD-L2/B7-DC/CD273. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1600 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for PDCD1LG2 elisa pair set

Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.

This PD-L2 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human PD-L2. It contains Human PD-L2 capture antibody, Human PD-L2 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human PD-L2 protein.

Product Categories/Family for PDCD1LG2 elisa pair set

Cancer Drug Targets

References

Latchman Y, et al. (2001) PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2: 261-8.Carreno BM, et al. (2005) Therapeutic opportunities in the B7/CD28 family of ligands and receptors. Curr Opin Pharmacol. 5(4): 424-30.Radhakrishnan S, et al. (2007) B7-DC/PD-L2 cross-linking induces NF-kappaB-dependent protection of dendritic cells from cell death. J Immunol. 178(3): 1426-32.