Western Blot (WB)
(Western blot analysis of extracts from 293T, using hSET1 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit hSET1 Polyclonal Antibody | anti-SETD1A antibody
hSET1 Antibody
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Dog(100%)
ELISA(peptide): 1:20,000-1:40,000
Western Blot (WB)
(Western blot analysis of extracts from 293T, using hSET1 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from 293T, using hSET1 Antibody. The lane on the left was treated with blocking peptide.)
Subcellular Location: Nucleus speckle. Chromosome. Note: Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.
Subunit Structure: Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Interacts with HCFC1. Interacts with ASH2/ASH2L, CXXC1/CFP1 and RBBP5. Interacts (via the RRM domain) with WDR82. Interacts (via the RRM domain) with hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A) only in the presence of WDR82. Binds specifically to CTD heptad repeats phosphorylated on 'Ser-5' of each heptad. Interacts with ZNF335. Interacts with SUPT6H. Interacts with NAP1L1 (By similarity). Interacts (via WIN motif) with WDR5 (PubMed:17998332, PubMed:22665483, PubMed:22266653).
Similarity: Belongs to the class V-like SAM-binding methyltransferase superfamily.
NCBI and Uniprot Product Information
Predicted Molecular Weight: (Calculated)186kDa.
NCBI Description
The protein encoded by this gene is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to generally mark the transcription start sites of active genes. The protein contains SET domains, a RNA recognition motif domain and is a member of the class V-like SAM-binding methyltransferase superfamily. [provided by RefSeq, Dec 2016]
Uniprot Description
SETD1A: Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys- 9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
Protein type: Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: histone methyltransferase complex; nuclear chromatin; nuclear speck; nucleoplasm; nucleus
Molecular Function: beta-catenin binding; histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; protein binding; transcription factor binding
Research Articles on SETD1A
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Product Notes
The SETD1A setd1a (Catalog #AAA9612141) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The hSET1 Antibody reacts with Human Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Dog(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's hSET1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the SETD1A setd1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "hSET1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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