Western Blot (WB)
(Detection of TTK with AF6028. Lysates from human A431, Daudi and MCF-7 cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 0.5 μg/mL anti-TTK, as described in Protocols for IWestern Blot)
Sheep anti-Human TTK Polyclonal Antibody | anti-ttk antibody
TTK (TTK Protein Kinase, Dual Specificity Protein Kinase TTK, ECSTY Kinase, ESK, FLJ38280, hMPS1, Monopolar Spindle 1 Like 1, MPS1L1, Mps1, Phosphotyrosine Picked Threonine Kinase, PYT)
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
TTK; Polyclonal Antibody; TTK (TTK Protein Kinase; Dual Specificity Protein Kinase TTK; ECSTY Kinase; ESK; FLJ38280; hMPS1; Monopolar Spindle 1 Like 1; MPS1L1; Mps1; Phosphotyrosine Picked Threonine Kinase; PYT); Anti -TTK (TTK Protein Kinase; anti-ttk antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous human TTK at ~100kD.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophillized powder in PBS, 5% Trehalose. Reconstitute with sterile 40-50% glycerol, PBS, 0.02% sodium azide.
Applicable Applications for anti-ttk antibody
Western Blot (WB)
Application Notes
Dilution: Western Blot: 0.5ug/ml
Immunogen
E. coli-derived rhTTK (aa 281-481).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Detection of TTK with AF6028. Lysates from human A431, Daudi and MCF-7 cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 0.5 μg/mL anti-TTK, as described in Protocols for IWestern Blot)
Western Blot (WB)
(Detection of TTK with AF6028. Lysates from human A431, Daudi and MCF-7 cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 0.5 μg/mL anti-TTK, as described in Protocols for IWestern Blot)
Related Product Information for anti-ttk antibody
TTK (Dual specificity Thr/Tyr kinase; also PYT and hMps1) is a 97-105kD member of the Ser/Thr protein kinase family of enzymes. It is expressed in rapidly proliferating tissues and cell lines, and participates in cell cycle regulation. In particular, it directs chromosome alignment, centrosome duplication, and spindle assembly checkpoint formation. Via phosphorylation, TTK is known to regulate p53, CDCA8 and TACC2. Human TTK is 857 (aa) in length and contains one protein kinase domain (aa525-791). Activation occurs following homodimerization and autophosphorylation on Thr675, Thr676 and Thr686.
Product Categories/Family for anti-ttk antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
TTK protein kinase
NCBI Official Synonym Full Names
TTK protein kinase
NCBI Official Symbol
ttk
NCBI Protein Information
TTK protein kinase
Protein Family
Similar Products
Product Notes
The ttk (Catalog #AAA623991) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The TTK (TTK Protein Kinase, Dual Specificity Protein Kinase TTK, ECSTY Kinase, ESK, FLJ38280, hMPS1, Monopolar Spindle 1 Like 1, MPS1L1, Mps1, Phosphotyrosine Picked Threonine Kinase, PYT) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TTK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Dilution: Western Blot: 0.5ug/ml. Researchers should empirically determine the suitability of the ttk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TTK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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