SDS-Page
(Detection of CD1c with MBS620009. Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml goat anti-CD1c.)
Goat anti-Human CD1c Polyclonal Antibody | anti-CD1C antibody
CD1c (Cluster of differentiation antigen 1c, CD1c antigen, CD1c antigen c polypeptide, CD1c molecule, Cortical thymocyte antigen CD1c, Differentiation antigen CD1 alpha 3, R7, T cell surface glycoprotein CD1c)
Gene Names
CD1C; R7; CD1; CD1A; BDCA1; CD1C
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
CD1c; Polyclonal Antibody; CD1c (Cluster of differentiation antigen 1c; CD1c antigen; CD1c antigen c polypeptide; CD1c molecule; Cortical thymocyte antigen CD1c; Differentiation antigen CD1 alpha 3; R7; T cell surface glycoprotein CD1c); Anti -CD1c (Cluster of differentiation antigen 1c; anti-CD1C antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CD1c.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS.
Applicable Applications for anti-CD1C antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Immunogen
NS0-derived recombinant human CD1c (aa19-302; Accession # P29017, with a Phe300Ser substitution).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of CD1c with MBS620009. Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml goat anti-CD1c.)
SDS-Page
(Detection of CD1c with MBS620009. Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml goat anti-CD1c.)
Related Product Information for anti-CD1C antibody
CD1c (Cluster of differentiation antigen 1c) is a 43kD member of the CD1 family of molecules. It is expressed by thymocytes, dendritic cells and B cells, and exists as part of a noncovalent complex with 12kD B2-microglobulin. It is found in the plasma membrane and early endosomes (but not lysosomes), and is presumed to present glycolipids and acylated peptides to T cells. Mature human CD1c is a 316aa type I transmembrane glycoprotein. It contains a 285aa extracellular domain (ECD) (aa18-302) plus a 10aa cytoplasmic tail. The ECD shows one Ig-like domain (aa203-296) that associates with B2-microglobulin, and a TyrGlnAspIle internalization motif in the cytoplasmic tail. There are three potential splice variants. One shows a Trp substitution for aa327-333, a second shows an eight aa substitution for aa298-333, and a third shows a 50aa substitution for aa297-333. There appears to be no direct mouse counterpart to human CD1c.
Product Categories/Family for anti-CD1C antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
CD1C
NCBI Official Synonym Full Names
CD1c molecule
NCBI Official Symbol
CD1C
NCBI Official Synonym Symbols
R7; CD1; CD1A; BDCA1; CD1C
NCBI Protein Information
T-cell surface glycoprotein CD1c; OTTHUMP00000035066; CD1C antigen, c polypeptide; cortical thymocyte antigen CD1C; differentiation antigen CD1-alpha-3
UniProt Protein Name
T-cell surface glycoprotein CD1c
Protein Family
UniProt Entry Name
CD1C_HUMAN
NCBI Description
This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene is broadly distributed throughout the endocytic system via a tyrosine-based motif in the cytoplasmic tail. Alternatively spliced transcript variants of this gene have been observed, but their full-length nature is not known. [provided by RefSeq]
Uniprot Description
CD1C: Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q23.1
Cellular Component: integral to plasma membrane; plasma membrane; endosome membrane
Molecular Function: beta-2-microglobulin binding; exogenous lipid antigen binding; endogenous lipid antigen binding; glycolipid binding
Biological Process: antigen processing and presentation, exogenous lipid antigen via MHC class Ib; T cell activation during immune response
Research Articles on CD1C
1. A model of CD1c with bound mannosyl-beta(1)-phosphomycoketide was constructed and analyzed through molecular dynamics simulations.
2. Data show that CD1c represents the second member of the CD1 family to present lipopeptides.
3. Microsomal triglyceride transfer protein regulates endogenous and exogenous antigen presentation by group 1 CD1 molecules.
4. monocyte-derived DCs cultured in an immunoglobulin-rich milieu expressed CD1d but not CD1a, CD1b, and CD1c, whereas DCs cultured in the presence of low levels of immunoglobulins had an opposite CD1 profile
5. HCMV encodes multiple blocking strategies targeting group 1 CD1 molecules CD1a, CD1b, and CD1c
6. Observational study of gene-disease association. (HuGE Navigator)
7. CD1c expression was detected on monocytes in the majority of sickle cell anemia patients, and was highly expressed in Sbeta thalassemia patients.
8. CD1b and CD1c show strong binding of nitrobenzoxadiazole (NBD)-labeled dialkyl-based ligands.
9. hypothesis that CD1-restricted T cells might be activated and home to target tissues involved in Hashimoto's thyroiditis and Graves' disease
10. identified CD1a-, CD1b-, and CD1c-restricted T cells from normal human donors that induce cytolysis and secrete copious IFN-gamma in response to self-CD1 expressed on monocyte-derived dendritic cells
2. Data show that CD1c represents the second member of the CD1 family to present lipopeptides.
3. Microsomal triglyceride transfer protein regulates endogenous and exogenous antigen presentation by group 1 CD1 molecules.
4. monocyte-derived DCs cultured in an immunoglobulin-rich milieu expressed CD1d but not CD1a, CD1b, and CD1c, whereas DCs cultured in the presence of low levels of immunoglobulins had an opposite CD1 profile
5. HCMV encodes multiple blocking strategies targeting group 1 CD1 molecules CD1a, CD1b, and CD1c
6. Observational study of gene-disease association. (HuGE Navigator)
7. CD1c expression was detected on monocytes in the majority of sickle cell anemia patients, and was highly expressed in Sbeta thalassemia patients.
8. CD1b and CD1c show strong binding of nitrobenzoxadiazole (NBD)-labeled dialkyl-based ligands.
9. hypothesis that CD1-restricted T cells might be activated and home to target tissues involved in Hashimoto's thyroiditis and Graves' disease
10. identified CD1a-, CD1b-, and CD1c-restricted T cells from normal human donors that induce cytolysis and secrete copious IFN-gamma in response to self-CD1 expressed on monocyte-derived dendritic cells
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Product Notes
The CD1C (Catalog #AAA620009) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The CD1c (Cluster of differentiation antigen 1c, CD1c antigen, CD1c antigen c polypeptide, CD1c molecule, Cortical thymocyte antigen CD1c, Differentiation antigen CD1 alpha 3, R7, T cell surface glycoprotein CD1c) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD1c can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 0.5-1ug/ml Western Blot: 1ug/ml. Researchers should empirically determine the suitability of the CD1C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD1c, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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