Flow Cytometry (FC/FACS)
(Figure 1a: Flow cytometric analysis of the BrdU-labeled fraction in a tissue cell culture.)
Mouse Bromodeoxyuridine / BrdU Monoclonal Antibody
Mouse anti Bromodeoxyuridine / BrdU
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry
Synonyms
Bromodeoxyuridine / BrdU; Monoclonal Antibody; Mouse anti Bromodeoxyuridine / BrdU; anti-Bromodeoxyuridine / BrdU antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
IIB5
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-Bromodeoxyuridine / BrdU antibody
Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin)
Application Notes
IIB5 is suitable for flow cytometry and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent.
Source Note
IIB5 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse intraperitoneally immunized with BrdU conjugated to Bovine Serum albumin.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Flow Cytometry (FC/FACS)
(Figure 1a: Flow cytometric analysis of the BrdU-labeled fraction in a tissue cell culture.)
Flow Cytometry (FC/FACS)
(Figure 1a: Flow cytometric analysis of the BrdU-labeled fraction in a tissue cell culture.)
Flow Cytometry (FC/FACS)
(Figure 1b: Flow cytometric analysis of the BrdU-labeled fraction in a tissue cell culture.)
Flow Cytometry (FC/FACS)
(Figure 1b: Flow cytometric analysis of the BrdU-labeled fraction in a tissue cell culture.)
Immunofluorescence (IF)
(Figure 2: Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells )
Immunofluorescence (IF)
(Figure 2: Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells )
Related Product Information for anti-Bromodeoxyuridine / BrdU antibody
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorpoRated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorpoRated BrdU with specific anti-BrdU monoclonal antibodies is an accuRate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorpoRated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic prolifeRative information (such as the S-phase transit Rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Product Categories/Family for anti-Bromodeoxyuridine / BrdU antibody
References
Schutte, B., Reynders, M. M., van Assche, C. L., Hupperets, P. S., Bosman, F. T., and Blijham, G. H. (1987). An improved method for the immunocytochemical detection of bromodeoxyuridine labeled nuclei using flow cytometry, Cytometry 8, 372-6. Tinnemans, M. M., Schutte, B., Lenders, M. H., Ten Velde, G. P., Ramaekers, F. C., and Blijham, G. H. (1993). Cytokinetic analysis of lung cancer by in vivo bromodeoxyuridine labelling, Br J Cancer 67, 1217-22. Schutte, B., Tinnemans M.M.F.J., Pijpers, G.F.P., Lenders, M.J.H. and Ramaekers, F. (1995). Three parameter flow cytometric analysis for simultaneous detection of cytokeRatin, prolifeRation associated antigens and DNA content. Cytometry 21, 177-86. van Engeland, M., Kuijpers, H. J., Ramaekers, F. C., Reutelingsperger, C. P., and Schutte, B. (1997). Plasma membrane alteRations and cytoskeletal changes in apoptosis. Exp Cell Res 235, 421-430. Schutte, B., Nieland, L., van Engeland, M., Henfling, M. E., Meijer, L., and Ramaekers, F. C. (1997). The effect of the cyclin-dependent kinase inhibitor olomoucine on cell cycle kinetics. Exp Cell Res 236, 4-15.
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Product Notes
The Bromodeoxyuridine / BrdU (Catalog #AAA570077) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Bromodeoxyuridine / BrdU can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin). IIB5 is suitable for flow cytometry and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent. Researchers should empirically determine the suitability of the Bromodeoxyuridine / BrdU for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bromodeoxyuridine / BrdU, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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