Testing Data
(Figure 1: Immunohistochemistry on frozen section of rabbit heart
)
Mouse anti-Human, Swine Cardiotin Monoclonal Antibody
Mouse anti Cardiotin
Reactivity
Human, Swine
Applications
Immunohistochemistry, Western Blot
Synonyms
Cardiotin; Monoclonal Antibody; Mouse anti Cardiotin; anti-Cardiotin antibody
Host
Mouse
Reactivity
Human, Swine
Clonality
Monoclonal
Isotype
IgM
Clone Number
SR-4
Specificity
SR-4 reacts with cardiotin, a mitochondrion-associated protein, which is present in cardiomyocytes and skeletal muscle, but also in epithelial cells and tissues.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-Cardiotin antibody
Immunohistochemistry (IHC) (frozen), Western Blot (WB)
Application Notes
SR-4 is useful for immunohistochemistry on frozen tissue sections and immunoblotting. In immunoblotting assays SR-4 reacts exclusively with the 300 kDa cardiotin protein complex. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:50 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:25 - 1:250 for immunoblotting appliCations.
Source Note
SR-4 is a Mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with cardiotin.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Testing Data
(Figure 1: Immunohistochemistry on frozen section of rabbit heart )
Testing Data
(Figure 1: Immunohistochemistry on frozen section of rabbit heart
)
Related Product Information for anti-Cardiotin antibody
Cardiotin is a high molecular weight protein complex (300 kDa) loCated in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuRation. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to Human skeletal muscle alpha-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.
Product Categories/Family for anti-Cardiotin antibody
References
1. No references available
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Product Notes
The Cardiotin (Catalog #AAA570090) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Cardiotin reacts with Human, Swine and may cross-react with other species as described in the data sheet. AAA Biotech's Cardiotin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) (frozen), Western Blot (WB). SR-4 is useful for immunohistochemistry on frozen tissue sections and immunoblotting. In immunoblotting assays SR-4 reacts exclusively with the 300 kDa cardiotin protein complex. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:50 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:25 - 1:250 for immunoblotting appliCations. Researchers should empirically determine the suitability of the Cardiotin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cardiotin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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