Mouse BrdU Monoclonal Antibody | anti-BrdU antibody

MOUSE ANTI BrdU

Cat. Num. AAA216162

• Applications: Immunohistochemistry, Flow Cytometry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
• Synonyms: BrdU; Monoclonal Antibody; MOUSE ANTI BrdU; anti-BrdU antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: Bu20a
• Form/Format: Purified; Purified IgG - Liquid; Phosphate buffered saline
• Concentration: IgG concentration 1.0mg/ml (varies by lot)

• Applicable Applications for anti-BrdU antibody: Immunohistology Frozen, Flow cytometry (FC), Immunohistology Paraffin, Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: Flow Cytometry : Use 10uL of the suggested working dilution to label 1X10⁶ cells in 100uL; Flow Cytometry: 1/25-1/100; Immunohistology - Paraffin: 1/25-1/100
• Carrier Free: Yes
• Fusion Partners: Spleen cells from immunized Balb/c mice were fused cells of the NS1 myeloma cell line.
• Immunogen: Bromodeoxyuridine conjugated to BSA
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Preperation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Target Species: Chemical
• Preparation and Storage: This product is shipped at ambient tempeture. It is recommended to aliqout and store at -20°C on receipt. When thawed , aliquot the sample as needed. Keep aliquots at 2°C to 8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Testing Data

(Detection of BrdU incorporation into pulsed-labelled ACNC12 cells using Mouse anti-BrdU antibody (clone Bu20a, MBS212833))

Related Product Information for anti-BrdU antibody

Mouse anti BrdU antibody, clone Bu20a recognizes the thymidine analogue bromodoxyuridine (BrdU), which can be incorporated into DNA during S-phase of the cell cycle. The BU20a antibody is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques.


Specificity: BrdU; Mouse anti BrdU antibody, clone Bu20a recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which is incorporated to new DNA during replication instead of thymidine. BrdU can therefore be used to identify newly synthesized DNA. Mouse anti BrdU antibody, clone Bu20a, recognizes BrdU and other thymidine analogs; 5'-chloro-2'-deoxyuridine (CldU), 5'-iodo-2'-deoxyuridine (IdU) and 2'-deoxy-5-ethynyluridine (EdU), but only shows minimal reactivity with thymidine itself (Aten et al. 1992, Liboska et al. 2012, Magaud et al. 1989). Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004). BrdU can be used as a thymidine analog in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants. Mouse anti BrdU antibody, clone Bu20a, is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques (Makarev and Gorivodsky 2014).