Immunohistochemistry (IHC)
(Figure 1. Immunohistochemistry on H82 cells, derived from small cell lung cancer, showing strong positive staining for NCAM/CD56 at the sites of intracellular connections. Dilution 1:500.)
Mouse anti-Human, Mouse NCAM / CD56 Monoclonal Antibody
Mouse anti NCAM / CD56
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry
Synonyms
NCAM / CD56; Monoclonal Antibody; Mouse anti NCAM / CD56; anti-NCAM / CD56 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
RNL-1
Specificity
RNL-1 was defined as a cluster I antibody during the Second International Workshop on Small Cell Lung Cancer (SCLC) Antibodies. RNL-1 recognizes NCAM which is present in small cell lung cancer (SCLC) and lung carcinoids.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-NCAM / CD56 antibody
Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen)
Application Notes
RNL-1 is suitable for immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent.
Source Note
RNL-1 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with the small cell lung cancer cell line NCI-H82.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Immunohistochemistry (IHC)
(Figure 1. Immunohistochemistry on H82 cells, derived from small cell lung cancer, showing strong positive staining for NCAM/CD56 at the sites of intracellular connections. Dilution 1:500.)
Immunohistochemistry (IHC)
(Figure 1. Immunohistochemistry on H82 cells, derived from small cell lung cancer, showing strong positive staining for NCAM/CD56 at the sites of intracellular connections. Dilution 1:500.)
Related Product Information for anti-NCAM / CD56 antibody
NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be geneRated by alternative splicing and by posttranslational modifiCations, such as sialylation. During sialylation, polysialic acid (PSA) carbohydRates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins.
Product Categories/Family for anti-NCAM / CD56 antibody
References
Broers, J. L., Mijnheere, E. P., Rot, M. K., Schaart, G., Sijlmans, A., Boerman, O. C., and Ramaekers, F. C. (1991). Novel antigens characteristic of neuroendocrine malignancies, Cancer 67, 619-33. Mijnheere, E. P., Boerman, O. C., Broers, J. L., Klein Rot, M., Vooijs, G. P., and Ramaekers, F. C. (1991). Immunotargeting of Human small cell lung cancer xenografts in athymic mice using a monoclonal antibody (RNL-1) anti a neuroendocrine- related antigen, Br J Cancer Suppl 14, 78-81. Boerman, O. C., Mijnheere, E. P., Broers, J. L., Vooijs, G. P., and Ramaekers, F. C. (1991). Biodistribution of a monoclonal antibody (RNL-1) anti the neural cell adhesion molecule (NCAM) in athymic mice bearing Human small-cell lung-cancer xenografts, Int J Cancer 48, 457-62. Mijnheere, E. P., Boerman, O. C., Poels, L. G., Slobbe, R. L., van Eys, G. J. J. M., and Ramaekers, F. C. S. (1996). Pharmacokinetics, biodistribution and dosimetry of anti-NCAM monoclonal antibody RNL-1 and its fragments in experimental lung cancer, Journal of Experimental and Clinical Cancer Research 15, 11-18. Hens, J., Nuydens, R., Geerts, H., Senden, N. H., Van de Ven, W. J., Roebroek, A. J., van de Velde, H. J., Ramaekers, F. C., and Broers, J. L. (1998). Neuronal differentiation is accompanied by NSP-C expression, Cell Tissue Res 292, 229-37.
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Product Notes
The NCAM / CD56 (Catalog #AAA570106) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti NCAM / CD56 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NCAM / CD56 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen). RNL-1 is suitable for immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent. Researchers should empirically determine the suitability of the NCAM / CD56 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCAM / CD56, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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