Testing Data
(Figure 1: Immunofluorescence staining of a 7 days old zebrafish embryo
)
Mouse Desmin Monoclonal Antibody | anti-DES antibody
Mouse anti Desmin
Gene Names
DES; CSM1; CSM2; CMD1I; FLJ12025; FLJ39719; FLJ41013; FLJ41793
Reactivity
Canine, Chicken, Hamster, Human, Mouse, Rabbit, Rat, Swine, Zebrafish
Applications
Immunohistochemistry, Western Blot
Synonyms
Desmin; Monoclonal Antibody; Mouse anti Desmin; anti-DES antibody
Host
Mouse
Reactivity
Canine, Chicken, Hamster, Human, Mouse, Rabbit, Rat, Swine, Zebrafish
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
RD301
Specificity
RD301 reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-DES antibody
Immunohistochemistry (IHC) (frozen), Western Blot (WB)
Application Notes
RD301 is suitable for immunoblotting and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.
Source Note
RD301 is a Mouse monoclonal IgG2b antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a cytoskeletal desmin extract of Chicken gizzard.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Testing Data
(Figure 1: Immunofluorescence staining of a 7 days old zebrafish embryo )
Testing Data
(Figure 1: Immunofluorescence staining of a 7 days old zebrafish embryo
)
Related Product Information for anti-DES antibody
Desmin is a 53 kDa intermediate filament protein that exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.
Product Categories/Family for anti-DES antibody
References
Quax, W., van den Broek, L., Egberts, W. V., Ramaekers, F., and Bloemendal, H. (1985). Characterization of the Hamster desmin gene: expression and formation of desmin filaments in nonmuscle cells after gene transfer, Cell 43, 327-38. Verhagen, A. P., Aalders, T. W., Ramaekers, F. C., Debruyne, F. M., and Schalken, J. A. (1988). Differential expression of keRatins in the basal and luminal compartments of Rat prostatic epithelium during degeneRation and regeneRation, Prostate 13, 25-38. Pieper, F. R., Schaart, G., Krimpenfort, P. J., Henderik, J. B., Moshage, H. J., van de Kemp, A., Ramaekers, F. C., Berns, A., and Bloemendal, H. (1989). Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells, J Cell Biol 108, 1009-24. Schaart, G., Viebahn, C., Langmann, W., and Ramaekers, F. (1989). Desmin and titin expression in early postimplantation Mouse embryos, Development 107, 585-96. 5. Raats, J. M., Pieper, F. R., Vree Egberts, W. T., Verrijp, K. N., Ramaekers, F. C., and Bloemendal, H. (1990). Assembly of amino-terminally deleted desmin in vimentin-free cells, J Cell Biol 111, 1971-85. Schaart, G., Pieper, F. R., Kuijpers, H. J., Bloemendal, H., and Ramaekers, F. C. (1991). Baby Hamster kidney (BHK-21/C13) cells can express striated muscle type proteins, Differentiation 46, 105-15. Dispersyn, G. D., Geuens, E., Ver Donck, L., Ramaekers, F. C., and Borgers, M. (2001). Adult Rabbit cardiomyocytes undergo hibernation-like dedifferentiation when co-cultured with cardiac fibroblasts, Cardiovasc Res 51, 230-40. Council, L., Hameed, O. (2009). Differential expression of immunohistochemical markers in bladder smooth muscle and myofibroblasts, and the potential utility of desmin, smoothelin, and vimentin in staging of bladder carcinoma Modern Pathology 22, 639-50.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53,536 Da
NCBI Official Full Name
desmin
NCBI Official Synonym Full Names
desmin
NCBI Official Symbol
DES
NCBI Official Synonym Symbols
CSM1; CSM2; CMD1I; FLJ12025; FLJ39719; FLJ41013; FLJ41793
NCBI Protein Information
desmin; OTTHUMP00000064865; intermediate filament protein
UniProt Protein Name
Desmin
Protein Family
UniProt Gene Name
DES
UniProt Entry Name
DESM_HUMAN
NCBI Description
This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq]
Uniprot Description
desmin: a class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: intermediate filament; fascia adherens; cytosol; neuromuscular junction; Z disc; sarcolemma
Molecular Function: identical protein binding; protein binding; structural constituent of cytoskeleton; cytoskeletal protein binding
Biological Process: muscle contraction; cytoskeleton organization and biogenesis; regulation of heart contraction; muscle filament sliding
Disease: Cardiomyopathy, Dilated, 1i; Scapuloperoneal Syndrome, Neurogenic, Kaeser Type; Myopathy, Myofibrillar, 1; Muscular Dystrophy, Limb-girdle, Type 2r
Research Articles on DES
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Product Notes
The DES des (Catalog #AAA570025) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Desmin reacts with Canine, Chicken, Hamster, Human, Mouse, Rabbit, Rat, Swine, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's Desmin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) (frozen), Western Blot (WB). RD301 is suitable for immunoblotting and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations. Researchers should empirically determine the suitability of the DES des for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Desmin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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