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Immunohistochemistry (IHC) (Figure 1. immunohistochemistry on frozen section of human liver bile duct epithelium.)

Mouse CytokeRatin 19 / KeRatin K19 Monoclonal Antibody

Mouse anti CytokeRatin 19 / KeRatin K19

Reactivity
Human, Zebrafish
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
Synonyms
CytokeRatin 19 / KeRatin K19; Monoclonal Antibody; Mouse anti CytokeRatin 19 / KeRatin K19; anti-CytokeRatin 19 / KeRatin K19 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Zebrafish
Clonality
Monoclonal
Isotype
IgG1
Clone Number
RCK108
Specificity
RCK108 reacts exclusively with cytokeRatin 19 which is present in glandular-type epithelia and most carcinomas. Does not react with hepatocytes and hepatocellular carcinoma.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-CytokeRatin 19 / KeRatin K19 antibody
Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
Application Notes
RCK108 is particularly well suited for use in immunohistochemistry on paraffin-embedded tissues after pretreatment with pepsin or trypsin. RCK108 is also useful for immunocytochemistry, immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.
Source Note
RCK108 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a cytoskeletal prepaRation of Human bladder carcinoma cell line T24.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.

Immunohistochemistry (IHC)

(Figure 1. immunohistochemistry on frozen section of human liver bile duct epithelium.)

Immunohistochemistry (IHC) (Figure 1. immunohistochemistry on frozen section of human liver bile duct epithelium.)

Immunofluorescence (IF)

(Figure 2. immunofluorescence staining of a 9 days old zebrafish embryo.)

Immunofluorescence (IF) (Figure 2. immunofluorescence staining of a 9 days old zebrafish embryo.)

Immunohistochemistry (IHC)

(Figure 3. Immunohistochemistry on a frozen tissue sections of human kidney (RCK108; dilution 1:500).)

Immunohistochemistry (IHC) (Figure 3. Immunohistochemistry on a frozen tissue sections of human kidney (RCK108; dilution 1:500).)

Immunohistochemistry (IHC)

(Figure 4. Immunohistochemistry on frozen tissue sections of human colon with (RCK108; dilution 1:500).)

Immunohistochemistry (IHC) (Figure 4. Immunohistochemistry on frozen tissue sections of human colon with (RCK108; dilution 1:500).)

Testing Data

(Figure 5. Indirect immunoperoxidase staining of human small intestine paraffin tissue section (RCK108; Mouse anti keratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.)

Testing Data (Figure 5. Indirect immunoperoxidase staining of human small intestine paraffin tissue section (RCK108; Mouse anti keratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.)

Testing Data

(Figure 6. Indirect immunoperoxidase staining of human placenta paraffin tissue section (RCK108; Mouse anti keratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.)

Testing Data (Figure 6. Indirect immunoperoxidase staining of human placenta paraffin tissue section (RCK108; Mouse anti keratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.)

Testing Data

(Figure 7. Indirect immunoperoxidase staining of human colon adenocarcinoma paraffin tissue section (RCK108; Mouse anti keratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial tumor cells. No reactivity in connective tissues.)

Testing Data (Figure 7. Indirect immunoperoxidase staining of human colon adenocarcinoma paraffin tissue section (RCK108; Mouse anti keratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial tumor cells. No reactivity in connective tissues.)
Related Product Information for anti-CytokeRatin 19 / KeRatin K19 antibody
CytokeRatins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual Human cytokeRatins are numbered 1 to 20. The various epithelia in the Human body usually express cytokeRatins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. CytokeRatin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeRatin antibodies are not only of assistance in the differential identification of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Product Categories/Family for anti-CytokeRatin 19 / KeRatin K19 antibody
References
Kwaspen, F. H., Smedts, F. M., Broos, A., Bulten, H., Debie, W. M., and Ramaekers, F. C. (1997). Reproducible and highly sensitive detection of the broad spectrum epithelial marker keRatin 19 in routine cancer diagnosis, Histopathology 31, 503-16.

NCBI and Uniprot Product Information

NCBI GI #
NCBI Official Full Name
cytokeratin 19

Uniprot Description

K19: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 19 differs from all other IF proteins in lacking the C-terminal tail domain. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: dystrophin-associated glycoprotein complex; costamere; intermediate filament; Z disc; sarcolemma

Molecular Function: protein binding; structural constituent of cytoskeleton; structural constituent of muscle; protein complex binding

Biological Process: viral reproduction; response to estrogen stimulus; sarcomere organization

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Product Notes

The CytokeRatin 19 / KeRatin K19 (Catalog #AAA570115) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti CytokeRatin 19 / KeRatin K19 reacts with Human, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's CytokeRatin 19 / KeRatin K19 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB). RCK108 is particularly well suited for use in immunohistochemistry on paraffin-embedded tissues after pretreatment with pepsin or trypsin. RCK108 is also useful for immunocytochemistry, immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations. Researchers should empirically determine the suitability of the CytokeRatin 19 / KeRatin K19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CytokeRatin 19 / KeRatin K19, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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