Mouse Neurofilament 200 kD Monoclonal Antibody

Mouse anti Neurofilament 200 kD

Cat. Num. AAA570127

• Reactivity: Bovine, Canine, Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus
• Applications: Immunohistochemistry, Immunohistochemistry, Western Blot
• Synonyms: Neurofilament 200 kD; Monoclonal Antibody; Mouse anti Neurofilament 200 kD; anti-Neurofilament 200 kD antibody

• Host: Mouse
• Reactivity: Bovine, Canine, Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: RNF402
• Specificity: RNF402 reacts with both the phosphorylated and non-phosphorylated isoform of the 200 kD neurofilament protein.
• Form/Format: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-Neurofilament 200 kD antibody: Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
• Application Notes: RNF402 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:10 - 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:25 - 1:250 for immunoblotting appliCations.
• Source Note: RNF402 is a Mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a neurofilament prepaRation of calf brain tissue.
• Preparation and Storage: Store at 4 degree C, or in small aliquots at -20 degree C.

Testing Data

(Figure 1: Immunohistochemistry on frozen section of rat brain )

Related Product Information for anti-Neurofilament 200 kD antibody

Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the Mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.

Product Categories/Family for anti-Neurofilament 200 kD antibody

Cytoskeletal; Cell differentiation; Cytoskeleton; Neurobiology; Stem cell research

References

Kuijpers, W., Tonnaer, E. L., Peters, T. A., and Ramaekers, F. C. (1991). Expression of intermediate filament proteins in the mature inner ear of the Rat and Guinea Pig, Hear Res 52, 133-46. Bauwens, L. J., De Groot, J. C., Ramaekers, F. C., Veldman, J. E., and Huizing, E. H. (1992). Expression of intermediate filament proteins in the adult Human vestibular labyrinth, Ann Otol Rhinol Laryngol 101, 479-86.