Testing Data
(Figure 1: Immunofluorescence staining of a 7 days old zebrafish embryo
)
Mouse Mitotic cells Monoclonal Antibody
Mouse anti Mitotic cells
Reactivity
Human, Zebrafish
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Synonyms
Mitotic cells; Monoclonal Antibody; Mouse anti Mitotic cells; anti-Mitotic cells antibody
Host
Mouse
Reactivity
Human, Zebrafish
Clonality
Monoclonal
Isotype
IgM
Clone Number
8B3G
Specificity
8B3G strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) 8B3G clearly distinguishes these M-phase cells from cell at other stages of the cell cycle (see figure). Dynamic information can be obtained by combining BrdU incorpoRation with 8B3G staining, which can distinguish and quantitate the four major fractions of the cell cycle.
Form/Format
Each vial contains 100 ul purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-Mitotic cells antibody
Flow Cytometry (FC), Immunocytochemistry (IHC)
Application Notes
8B3G can be used for flow cytometric analyses and immunocytochemistry. 8B3G is not suitable for immunoblotting. Optimal antibody dilution should be determined by titRation; recommended range is 1:50 - 1:100 for flow cytometry, and for immunocytochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent.
Source Note
8B3G is a Mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a total cell lysate of the Human bladder carcinoma cell line T24.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Testing Data
(Figure 1: Immunofluorescence staining of a 7 days old zebrafish embryo )
Testing Data
(Figure 1: Immunofluorescence staining of a 7 days old zebrafish embryo
)
Related Product Information for anti-Mitotic cells antibody
The life cycle of a eukaryotic cell consists of various phases, two of which can morphologically and biochemically be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is repliCated, a process that can be detected using biochemical techniques, such as the BrdU incorpoRation assay. In between the M- and S-phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA repliCation, and G2-phase, the gap between completion of DNA repliCation and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a 'quiescent' phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S, and from G2 to M-phase.
Product Categories/Family for anti-Mitotic cells antibody
References
1. No references available
Similar Products
Product Notes
The Mitotic cells (Catalog #AAA570109) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Mitotic cells reacts with Human, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's Mitotic cells can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunocytochemistry (IHC). 8B3G can be used for flow cytometric analyses and immunocytochemistry. 8B3G is not suitable for immunoblotting. Optimal antibody dilution should be determined by titRation; recommended range is 1:50 - 1:100 for flow cytometry, and for immunocytochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent. Researchers should empirically determine the suitability of the Mitotic cells for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mitotic cells, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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