Mouse CytokeRatin 7 / KeRatin K7 Monoclonal Antibody

Mouse anti CytokeRatin 7 / KeRatin K7

Cat. Num. AAA570028

• Reactivity: Canine, Feline, Caprine, Hamster, Human, Mouse, Rat, Zebrafish
• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
• Synonyms: CytokeRatin 7 / KeRatin K7; Monoclonal Antibody; Mouse anti CytokeRatin 7 / KeRatin K7; anti-CytokeRatin 7 / KeRatin K7 antibody

• Host: Mouse
• Reactivity: Canine, Feline, Caprine, Hamster, Human, Mouse, Rat, Zebrafish
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: RCK105
• Specificity: RCK105 reacts exclusively with cytokeRatin 7 which is present in a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma.
• Form/Format: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-CytokeRatin 7 / KeRatin K7 antibody: Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Western Blot (WB)
• Application Notes: RCK105 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.
• Source Note: RCK105 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with cytokeRatins from the Human bladder carcinoma cell line T24.
• Preparation and Storage: Store at 4 degree C, or in small aliquots at -20 degree C.

Immunohistochemistry (IHC)

(Figure 1 - Immunohistochemistry on frozen sections of swine liver showing positive staining in the epithelial cells lining the bile ducts and no reactivity in hepatocytes or connective tissue. Dilution 1:500)

Immunohistochemistry (IHC)

(Figure 2 - Immunohistochemistry on frozen sections of swine liver showing positive staining in the epithelial cells lining the bile ducts and no reactivity in hepatocytes or connective tissue. Dilution 1:500)

Related Product Information for anti-CytokeRatin 7 / KeRatin K7 antibody

CytokeRatins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual Human cytokeRatins are numbered 1 to 20. The various epithelia in the Human body usually express cytokeRatins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. CytokeRatin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeRatin antibodies are not only of assistance in the differential identification of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Product Categories/Family for anti-CytokeRatin 7 / KeRatin K7 antibody

Cytoskeletal; Cancer; Cytoskeleton

References

Ramaekers, F., Huysmans, A., Schaart, G., Moesker, O., and Vooijs, P. (1987). Tissue distribution of keRatin 7 as monitored by a monoclonal antibody, Exp Cell Res 170, 235-49.Smedts, F., Ramaekers, F., Robben, H., Pruszczynski, M., van Muijen, G., Lane, B., Leigh, I., and Vooijs, P. (1990). Changing patterns of keRatin expression during progression of cervical intraepithelial neoplasia, Am J Pathol 136, 657-68.Schaafsma, H. E., Ramaekers, F. C., van Muijen, G. N., Lane, E. B., Leigh, I. M., Robben, H., Huijsmans, A., Ooms, E. C., and Ruiter, D. J. (1990). Distribution of cytokeRatin polypeptides in Human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression, Am J Pathol 136, 329-43.Ramaekers, F., van Niekerk, C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G., and Vooijs, P. (1990). Use of monoclonal antibodies to keRatin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55.Bauwens, L. J., De Groot, J. C., Ramaekers, F. C., Veldman, J. E., and Huizing, E. H. (1992). Expression of intermediate filament proteins in the adult Human vestibular labyrinth, Ann Otol Rhinol Laryngol 101, 479-86.Smedts, F., Ramaekers, F., Troyanovsky, S., Pruszczynski, M., Link, M., Lane, B., Leigh, I., Schijf, C., and Vooijs, P. (1992). KeRatin expression in cervical cancer, Am J Pathol 141, 497-511.Ivanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., and van Doornewaard, G. (1992). Patterns of expression of feline cytokeRatins in healthy epithelia and mammary carcinoma cells, Am J Vet Res 53, 304-14.